Heterochromatin Protein 1 Secures Survival and Transmission of Malaria Parasites
Clonally variant expression of surface antigens allows the malaria parasite Plasmodium falciparum to evade immune recognition during blood stage infection and secure malaria transmission. We demonstrate that heterochromatin protein 1 (HP1), an evolutionary conserved regulator of heritable gene silen...
Saved in:
Main Authors: | , , , , , , , , , , |
---|---|
Other Authors: | |
Format: | Article |
Language: | English |
Published: |
2016
|
Subjects: | |
Online Access: | https://hdl.handle.net/10356/82524 http://hdl.handle.net/10220/40061 |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
Institution: | Nanyang Technological University |
Language: | English |
id |
sg-ntu-dr.10356-82524 |
---|---|
record_format |
dspace |
spelling |
sg-ntu-dr.10356-825242020-03-07T12:18:05Z Heterochromatin Protein 1 Secures Survival and Transmission of Malaria Parasites Brancucci, Nicolas M.B. Bertschi, Nicole L. Zhu, Lei Niederwieser, Igor Chin, Wai Hoe Wampfler, Rahel Freymond, Céline Rottmann, Matthias Felger, Ingrid Bozdech, Zbynek Voss, Till S. School of Biological Sciences Biological Sciences Clonally variant expression of surface antigens allows the malaria parasite Plasmodium falciparum to evade immune recognition during blood stage infection and secure malaria transmission. We demonstrate that heterochromatin protein 1 (HP1), an evolutionary conserved regulator of heritable gene silencing, controls expression of numerous P. falciparum virulence genes as well as differentiation into the sexual forms that transmit to mosquitoes. Conditional depletion of P. falciparum HP1 (PfHP1) prevents mitotic proliferation of blood stage parasites and disrupts mutually exclusive expression and antigenic variation of the major virulence factor PfEMP1. Additionally, PfHP1-dependent regulation of PfAP2-G, a transcription factor required for gametocyte conversion, controls the switch from asexual proliferation to sexual differentiation, providing insight into the epigenetic mechanisms underlying gametocyte commitment. These findings show that PfHP1 is centrally involved in clonally variant gene expression and sexual differentiation in P. falciparum and have major implications for developing antidisease and transmission-blocking interventions against malaria. NMRC (Natl Medical Research Council, S’pore) 2016-02-23T06:47:36Z 2019-12-06T14:57:18Z 2016-02-23T06:47:36Z 2019-12-06T14:57:18Z 2014 Journal Article Brancucci, N., Bertschi, N., Zhu, L., Niederwieser, I., Chin, W., Wampfler, R., Freymond, C., Rottmann, M., Felger, I., Bozdech, Z.,& Voss, T. (2014). Heterochromatin Protein 1 Secures Survival and Transmission of Malaria Parasites. Cell Host & Microbe, 16(2), 165-176. 1931-3128 https://hdl.handle.net/10356/82524 http://hdl.handle.net/10220/40061 10.1016/j.chom.2014.07.004 en Cell Host & Microbe © 2014 Elsevier Inc. |
institution |
Nanyang Technological University |
building |
NTU Library |
country |
Singapore |
collection |
DR-NTU |
language |
English |
topic |
Biological Sciences |
spellingShingle |
Biological Sciences Brancucci, Nicolas M.B. Bertschi, Nicole L. Zhu, Lei Niederwieser, Igor Chin, Wai Hoe Wampfler, Rahel Freymond, Céline Rottmann, Matthias Felger, Ingrid Bozdech, Zbynek Voss, Till S. Heterochromatin Protein 1 Secures Survival and Transmission of Malaria Parasites |
description |
Clonally variant expression of surface antigens allows the malaria parasite Plasmodium falciparum to evade immune recognition during blood stage infection and secure malaria transmission. We demonstrate that heterochromatin protein 1 (HP1), an evolutionary conserved regulator of heritable gene silencing, controls expression of numerous P. falciparum virulence genes as well as differentiation into the sexual forms that transmit to mosquitoes. Conditional depletion of P. falciparum HP1 (PfHP1) prevents mitotic proliferation of blood stage parasites and disrupts mutually exclusive expression and antigenic variation of the major virulence factor PfEMP1. Additionally, PfHP1-dependent regulation of PfAP2-G, a transcription factor required for gametocyte conversion, controls the switch from asexual proliferation to sexual differentiation, providing insight into the epigenetic mechanisms underlying gametocyte commitment. These findings show that PfHP1 is centrally involved in clonally variant gene expression and sexual differentiation in P. falciparum and have major implications for developing antidisease and transmission-blocking interventions against malaria. |
author2 |
School of Biological Sciences |
author_facet |
School of Biological Sciences Brancucci, Nicolas M.B. Bertschi, Nicole L. Zhu, Lei Niederwieser, Igor Chin, Wai Hoe Wampfler, Rahel Freymond, Céline Rottmann, Matthias Felger, Ingrid Bozdech, Zbynek Voss, Till S. |
format |
Article |
author |
Brancucci, Nicolas M.B. Bertschi, Nicole L. Zhu, Lei Niederwieser, Igor Chin, Wai Hoe Wampfler, Rahel Freymond, Céline Rottmann, Matthias Felger, Ingrid Bozdech, Zbynek Voss, Till S. |
author_sort |
Brancucci, Nicolas M.B. |
title |
Heterochromatin Protein 1 Secures Survival and Transmission of Malaria Parasites |
title_short |
Heterochromatin Protein 1 Secures Survival and Transmission of Malaria Parasites |
title_full |
Heterochromatin Protein 1 Secures Survival and Transmission of Malaria Parasites |
title_fullStr |
Heterochromatin Protein 1 Secures Survival and Transmission of Malaria Parasites |
title_full_unstemmed |
Heterochromatin Protein 1 Secures Survival and Transmission of Malaria Parasites |
title_sort |
heterochromatin protein 1 secures survival and transmission of malaria parasites |
publishDate |
2016 |
url |
https://hdl.handle.net/10356/82524 http://hdl.handle.net/10220/40061 |
_version_ |
1681040278265266176 |