The development of a single molecule fluorescence standard and its application in estimating the stoichiometry of the nuclear pore complex

We report here an image-based method to quantify the stoichiometry of diffraction-limited sub-cellular protein complexes in vivo under spinning disk confocal microscopy. A GFP single molecule fluorescence standard was first established by immobilizing His-tagged GFP molecules onto the glass surface...

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Bibliographic Details
Main Authors: Tie, Hieng Chiong, Madugula, Viswanadh, Lu, Lei
Other Authors: School of Biological Sciences
Format: Article
Language:English
Published: 2017
Subjects:
Online Access:https://hdl.handle.net/10356/83957
http://hdl.handle.net/10220/42890
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Institution: Nanyang Technological University
Language: English