The development of a single molecule fluorescence standard and its application in estimating the stoichiometry of the nuclear pore complex
We report here an image-based method to quantify the stoichiometry of diffraction-limited sub-cellular protein complexes in vivo under spinning disk confocal microscopy. A GFP single molecule fluorescence standard was first established by immobilizing His-tagged GFP molecules onto the glass surface...
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| Main Authors: | , , |
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| Other Authors: | |
| Format: | Article |
| Language: | English |
| Published: |
2017
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| Subjects: | |
| Online Access: | https://hdl.handle.net/10356/83957 http://hdl.handle.net/10220/42890 |
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| Institution: | Nanyang Technological University |
| Language: | English |