The development of a single molecule fluorescence standard and its application in estimating the stoichiometry of the nuclear pore complex

• Main Authors: Tie, Hieng Chiong, Madugula, Viswanadh, Lu, Lei
• Other Authors: School of Biological Sciences
• Format: Article
• Language: English
• Published: 2017
• Subjects: Stoichiometry; Single molecule
• Online Access: https://hdl.handle.net/10356/83957; http://hdl.handle.net/10220/42890
• Institution: Nanyang Technological University

We report here an image-based method to quantify the stoichiometry of diffraction-limited sub-cellular protein complexes in vivo under spinning disk confocal microscopy. A GFP single molecule fluorescence standard was first established by immobilizing His-tagged GFP molecules onto the glass surface...