Systematic evaluation of library preparation methods and sequencing platforms for high-throughput whole genome bisulfite sequencing

• Main Authors: Schuster, Stephan C., Beck, Stephan, Kim, Changhoon, Chambers, John Campbell, Loh, Marie, Zhou, Li, Ng, Hong Kiat, Drautz-Moses, Daniela I.
• 其他作者: Lee Kong Chian School of Medicine (LKCMedicine)
• 格式: Article
• 語言: English
• 出版: 2019
• 主題: Science::Biological sciences; Genomics; Biotechnology
• 在線閱讀: https://hdl.handle.net/10356/85180; http://hdl.handle.net/10220/49792

Whole genome bisulfite sequencing (WGBS), with its ability to interrogate methylation status at single CpG site resolution epigenome-wide, is a powerful technique for use in molecular experiments. Here, we aim to advance strategies for accurate and efficient WGBS for application in future large-scal...