Sulfinate Based Selective Labeling of 5‑Hydroxymethylcytosine: Application to Biotin Pull Down Assay

Sulfinate Based Selective Labeling of 5‑Hydroxymethylcytosine: Application to Biotin Pull Down Assay

We developed an enzyme-free, chemical method to selectively label the epigenetic base, 5-hydroxymethylcytosine (hmC) with versatile sulfinate reagents in aqueous solvent under mild reaction conditions. This method allows efficient single step conjugation of biotin to hmC site in DNA for enrichment a...

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Bibliographic Details
Main Authors: Wu, Qiong, Amrutkar, Suyog Madhav, Shao, Fangwei
Other Authors: School of Physical and Mathematical Sciences
Format: Article
Language:English
Published: 2018
Subjects:
5-Hydroxymethylcytosine
DNA
Online Access:https://hdl.handle.net/10356/88885
http://hdl.handle.net/10220/44746
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Institution: Nanyang Technological University
Language: English

Internet

https://hdl.handle.net/10356/88885
http://hdl.handle.net/10220/44746

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