Sulfinate Based Selective Labeling of 5‑Hydroxymethylcytosine: Application to Biotin Pull Down Assay
We developed an enzyme-free, chemical method to selectively label the epigenetic base, 5-hydroxymethylcytosine (hmC) with versatile sulfinate reagents in aqueous solvent under mild reaction conditions. This method allows efficient single step conjugation of biotin to hmC site in DNA for enrichment a...
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Main Authors: | Wu, Qiong, Amrutkar, Suyog Madhav, Shao, Fangwei |
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Other Authors: | School of Physical and Mathematical Sciences |
Format: | Article |
Language: | English |
Published: |
2018
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Subjects: | |
Online Access: | https://hdl.handle.net/10356/88885 http://hdl.handle.net/10220/44746 |
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Institution: | Nanyang Technological University |
Language: | English |
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