Sulfinate Based Selective Labeling of 5‑Hydroxymethylcytosine: Application to Biotin Pull Down Assay

Sulfinate Based Selective Labeling of 5‑Hydroxymethylcytosine: Application to Biotin Pull Down Assay

We developed an enzyme-free, chemical method to selectively label the epigenetic base, 5-hydroxymethylcytosine (hmC) with versatile sulfinate reagents in aqueous solvent under mild reaction conditions. This method allows efficient single step conjugation of biotin to hmC site in DNA for enrichment a...

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Main Authors: Wu, Qiong, Amrutkar, Suyog Madhav, Shao, Fangwei
Other Authors: School of Physical and Mathematical Sciences
Format: Article
Language:English
Published: 2018
Subjects:
5-Hydroxymethylcytosine
DNA
Online Access:https://hdl.handle.net/10356/88885
http://hdl.handle.net/10220/44746
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Institution: Nanyang Technological University
Language: English
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spelling sg-ntu-dr.10356-888852023-02-28T19:29:37Z Sulfinate Based Selective Labeling of 5‑Hydroxymethylcytosine: Application to Biotin Pull Down Assay Wu, Qiong Amrutkar, Suyog Madhav Shao, Fangwei School of Physical and Mathematical Sciences 5-Hydroxymethylcytosine DNA We developed an enzyme-free, chemical method to selectively label the epigenetic base, 5-hydroxymethylcytosine (hmC) with versatile sulfinate reagents in aqueous solvent under mild reaction conditions. This method allows efficient single step conjugation of biotin to hmC site in DNA for enrichment and pull down assays. MOE (Min. of Education, S’pore) Accepted version 2018-05-04T04:39:49Z 2019-12-06T17:13:03Z 2018-05-04T04:39:49Z 2019-12-06T17:13:03Z 2018 2018 Journal Article Wu, Q., Amrutkar, S. M., & Shao, F. (2018). Sulfinate Based Selective Labeling of 5‑Hydroxymethylcytosine: Application to Biotin Pull Down Assay. Bioconjugate Chemistry, 29(2), 245-249. 1043-1802 https://hdl.handle.net/10356/88885 http://hdl.handle.net/10220/44746 10.1021/acs.bioconjchem.7b00826 207084 en Bioconjugate Chemistry © 2018 American Chemical Society. This is the author created version of a work that has been peer reviewed and accepted for publication by Bioconjugate Chemistry, American Chemical Society. It incorporates referee’s comments but changes resulting from the publishing process, such as copyediting, structural formatting, may not be reflected in this document. The published version is available at: [http://dx.doi.org/10.1021/acs.bioconjchem.7b00826]. 18 p. application/pdf
institution Nanyang Technological University
building NTU Library
continent Asia
country Singapore
Singapore
content_provider NTU Library
collection DR-NTU
language English
topic 5-Hydroxymethylcytosine
DNA
spellingShingle 5-Hydroxymethylcytosine
DNA
Wu, Qiong
Amrutkar, Suyog Madhav
Shao, Fangwei
Sulfinate Based Selective Labeling of 5‑Hydroxymethylcytosine: Application to Biotin Pull Down Assay
description We developed an enzyme-free, chemical method to selectively label the epigenetic base, 5-hydroxymethylcytosine (hmC) with versatile sulfinate reagents in aqueous solvent under mild reaction conditions. This method allows efficient single step conjugation of biotin to hmC site in DNA for enrichment and pull down assays.
author2 School of Physical and Mathematical Sciences
author_facet School of Physical and Mathematical Sciences
Wu, Qiong
Amrutkar, Suyog Madhav
Shao, Fangwei
format Article
author Wu, Qiong
Amrutkar, Suyog Madhav
Shao, Fangwei
author_sort Wu, Qiong
title Sulfinate Based Selective Labeling of 5‑Hydroxymethylcytosine: Application to Biotin Pull Down Assay
title_short Sulfinate Based Selective Labeling of 5‑Hydroxymethylcytosine: Application to Biotin Pull Down Assay
title_full Sulfinate Based Selective Labeling of 5‑Hydroxymethylcytosine: Application to Biotin Pull Down Assay
title_fullStr Sulfinate Based Selective Labeling of 5‑Hydroxymethylcytosine: Application to Biotin Pull Down Assay
title_full_unstemmed Sulfinate Based Selective Labeling of 5‑Hydroxymethylcytosine: Application to Biotin Pull Down Assay
title_sort sulfinate based selective labeling of 5‑hydroxymethylcytosine: application to biotin pull down assay
publishDate 2018
url https://hdl.handle.net/10356/88885
http://hdl.handle.net/10220/44746
_version_ 1759856785559126016

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