Sulfinate Based Selective Labeling of 5‑Hydroxymethylcytosine: Application to Biotin Pull Down Assay

Sulfinate Based Selective Labeling of 5‑Hydroxymethylcytosine: Application to Biotin Pull Down Assay

We developed an enzyme-free, chemical method to selectively label the epigenetic base, 5-hydroxymethylcytosine (hmC) with versatile sulfinate reagents in aqueous solvent under mild reaction conditions. This method allows efficient single step conjugation of biotin to hmC site in DNA for enrichment a...

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Bibliographic Details
Main Authors: Wu, Qiong, Amrutkar, Suyog Madhav, Shao, Fangwei
Other Authors: School of Physical and Mathematical Sciences
Format: Article
Language:English
Published: 2018
Subjects:
5-Hydroxymethylcytosine
DNA
Online Access:https://hdl.handle.net/10356/88885
http://hdl.handle.net/10220/44746
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Institution: Nanyang Technological University
Language: English
Description
Summary:We developed an enzyme-free, chemical method to selectively label the epigenetic base, 5-hydroxymethylcytosine (hmC) with versatile sulfinate reagents in aqueous solvent under mild reaction conditions. This method allows efficient single step conjugation of biotin to hmC site in DNA for enrichment and pull down assays.

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