Single-molecule compaction of megabase-long chromatin molecules by multivalent cations

• Main Authors: Zinchenko, Anatoly, Berezhnoy, Nikolay V., Wang, Sal, Rosencrans, William M., Korolev, Nikolay, van der Maarel, Johan R. C., Nordenskiöld, Lars
• Other Authors: School of Biological Sciences
• Format: Article
• Language: English
• Published: 2018
• Subjects: Gene Regulation; Chromatin
• Online Access: https://hdl.handle.net/10356/89191; http://hdl.handle.net/10220/44813
• Institution: Nanyang Technological University

To gain insight into the conformational properties and compaction of megabase-long chromatin molecules, we reconstituted chromatin from T4 phage DNA (165 kb) and recombinant human histone octamers (HO). The unimolecular compaction, induced by divalent Mg2+ or tetravalent spermine4+ cations, studied...