Thermal proximity coaggregation for system-wide profiling of protein complex dynamics in cells

• Main Authors: Tan, Chris Soon Heng, Go, Ka Diam, Bisteau, Xavier, Dai, Lingyun, Yong, Chern Han, Prabhu, Nayana, Ozturk, Mert Burak, Lim, Yan Ting, Sreekumar, Lekshmy, Lengqvist, Johan, Tergaonkar, Vinay, Kaldis, Philipp, Sobota, Radoslaw M., Nordlund, Pär
• Other Authors: School of Biological Sciences
• Format: Article
• Language: English
• Published: 2019
• Subjects: DRNTU::Science::Biological sciences; Cellular Thermal Shift Assay; Protein Interaction Network
• Online Access: https://hdl.handle.net/10356/90293; http://hdl.handle.net/10220/48481
• Institution: Nanyang Technological University

Proteins differentially interact with each other across cellular states and conditions, but an efficient proteome-wide strategy to monitor them is lacking. We report the application of thermal proximity coaggregation (TPCA) for high-throughput intracellular monitoring of protein complex dynamics. Si...