Cloning, expression and characterization of the human transcription elongation factor, TFIIS

• Main Authors: Yoon, Ho Sup, Baek, Kwanghee, Jeon, Choon Ju, Miyamoto, Kenichi, Ueno, Akemichi, Agarwal, Kan, Yoo, Ook Joon
• Other Authors: School of Biological Sciences
• Format: Article
• Language: English
• Published: 2012
• Subjects: DRNTU::Science::Biological sciences::Biochemistry
• Online Access: https://hdl.handle.net/10356/94484; http://hdl.handle.net/10220/7658
• Institution: Nanyang Technological University

The cDNA for the human elongation factor, TFIIS, has been cloned and expressed in E. coil with the T7 expression system. This 280-amino acid TFIIS protein is shorter by 21 residues than that of the mouse. The missing 21 residues are located in the amino-terminal region, which is not thought to be re...