Cloning, expression and characterization of the human transcription elongation factor, TFIIS
The cDNA for the human elongation factor, TFIIS, has been cloned and expressed in E. coil with the T7 expression system. This 280-amino acid TFIIS protein is shorter by 21 residues than that of the mouse. The missing 21 residues are located in the amino-terminal region, which is not thought to be re...
Saved in:
Main Authors: | Yoon, Ho Sup, Baek, Kwanghee, Jeon, Choon Ju, Miyamoto, Kenichi, Ueno, Akemichi, Agarwal, Kan, Yoo, Ook Joon |
---|---|
Other Authors: | School of Biological Sciences |
Format: | Article |
Language: | English |
Published: |
2012
|
Subjects: | |
Online Access: | https://hdl.handle.net/10356/94484 http://hdl.handle.net/10220/7658 |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
Institution: | Nanyang Technological University |
Language: | English |
Similar Items
-
Stimulation of transcript elongation requires both the zinc finger and RNA polymerase II binding domains of human TFIIS
by: Agarwal, Kan, et al.
Published: (2012) -
Structure of a new nucleic-acid-binding motif in eukaryotic transcriptional elongation factor TFIIS
by: Weiss, Michael A., et al.
Published: (2012) -
The transcription factor TFIIS zinc ribbon dipeptide Asp-Glu is critical for stimulation of elongation and RNA cleavage by RNA polymerase II
by: Jeon, Choon Ju, et al.
Published: (2012) -
Novel zinc finger motif in the basal transcriptional machinery : three-dimensional NMR studies of the nucleic acid binding domain of transcriptional elongation factor TFIIS
by: Gozani, Shai N., et al.
Published: (2012) -
Preferential interaction of the mRNA proofreading factor TFIIS zinc ribbon with rU·dA base pairs correlates with its function
by: Sitikov, Albert S., et al.
Published: (2012)