Formation of pearl-necklace monomorphic G-quadruplexes in the human CEB25 minisatellite
CEB25 is a human minisatellite locus, composed of slightly polymorphic 52-nucleotide (nt) tandem repeats. Genetically, most if not all individuals of the human population are heterozygous, carrying alleles ranging from 0.5 to 20 kb, maintained by mendelian inheritance but also subject to germline in...
Saved in:
Main Authors: | , , , , , , |
---|---|
Other Authors: | |
Format: | Article |
Language: | English |
Published: |
2013
|
Online Access: | https://hdl.handle.net/10356/95807 http://hdl.handle.net/10220/11207 |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
Institution: | Nanyang Technological University |
Language: | English |
id |
sg-ntu-dr.10356-95807 |
---|---|
record_format |
dspace |
spelling |
sg-ntu-dr.10356-958072020-03-07T12:37:21Z Formation of pearl-necklace monomorphic G-quadruplexes in the human CEB25 minisatellite Amrane, Samir Adrian, Michael Heddi, Brahim Serero, Alexandre Nicolas, Alain Mergny, Jean-Louis Phan, Anh Tuân School of Physical and Mathematical Sciences CEB25 is a human minisatellite locus, composed of slightly polymorphic 52-nucleotide (nt) tandem repeats. Genetically, most if not all individuals of the human population are heterozygous, carrying alleles ranging from 0.5 to 20 kb, maintained by mendelian inheritance but also subject to germline instability. To provide insights on the biological role of CEB25, we interrogated its structural features. We report the NMR structure of the G-quadruplex formed by the conserved 26-nt G-rich fragment of the CEB25 motif. In K+ solution, this sequence forms a propeller-type parallel-stranded G-quadruplex involving a 9-nt central double-chain-reversal loop. This long loop is anchored to the 5′-end of the sequence by an A·T Watson–Crick base pair and a potential G·A noncanonical base pair. These base pairs contribute to the stability of the overall G-quadruplexstructure, as measured by an increase of about 17 kcal/mol in enthalpy or 6 °C in melting temperature. Further, we demonstrate that such a monomorphic structure is formed within longer sequence contexts folding into a pearl-necklace structure. 2013-07-11T06:25:28Z 2019-12-06T19:21:52Z 2013-07-11T06:25:28Z 2019-12-06T19:21:52Z 2012 2012 Journal Article Amrane, S., Adrian, M., Heddi, B., Serero, A., Nicolas, A., Mergny, J. L., et al. (2012). Formation of Pearl-Necklace Monomorphic G-Quadruplexes in the Human CEB25 Minisatellite. Journal of the American Chemical Society, 134(13), 5807-5816. https://hdl.handle.net/10356/95807 http://hdl.handle.net/10220/11207 10.1021/ja208993r en Journal of the American chemical society © 2012 American Chemical Society. |
institution |
Nanyang Technological University |
building |
NTU Library |
country |
Singapore |
collection |
DR-NTU |
language |
English |
description |
CEB25 is a human minisatellite locus, composed of slightly polymorphic 52-nucleotide (nt) tandem repeats. Genetically, most if not all individuals of the human population are heterozygous, carrying alleles ranging from 0.5 to 20 kb, maintained by mendelian inheritance but also subject to germline instability. To provide insights on the biological role of CEB25, we interrogated its structural features. We report the NMR structure of the G-quadruplex formed by the conserved 26-nt G-rich fragment of the CEB25 motif. In K+ solution, this sequence forms a propeller-type parallel-stranded G-quadruplex involving a 9-nt central double-chain-reversal loop. This long loop is anchored to the 5′-end of the sequence by an A·T Watson–Crick base pair and a potential G·A noncanonical base pair. These base pairs contribute to the stability of the overall G-quadruplexstructure, as measured by an increase of about 17 kcal/mol in enthalpy or 6 °C in melting temperature. Further, we demonstrate that such a monomorphic structure is formed within longer sequence contexts folding into a pearl-necklace structure. |
author2 |
School of Physical and Mathematical Sciences |
author_facet |
School of Physical and Mathematical Sciences Amrane, Samir Adrian, Michael Heddi, Brahim Serero, Alexandre Nicolas, Alain Mergny, Jean-Louis Phan, Anh Tuân |
format |
Article |
author |
Amrane, Samir Adrian, Michael Heddi, Brahim Serero, Alexandre Nicolas, Alain Mergny, Jean-Louis Phan, Anh Tuân |
spellingShingle |
Amrane, Samir Adrian, Michael Heddi, Brahim Serero, Alexandre Nicolas, Alain Mergny, Jean-Louis Phan, Anh Tuân Formation of pearl-necklace monomorphic G-quadruplexes in the human CEB25 minisatellite |
author_sort |
Amrane, Samir |
title |
Formation of pearl-necklace monomorphic G-quadruplexes in the human CEB25 minisatellite |
title_short |
Formation of pearl-necklace monomorphic G-quadruplexes in the human CEB25 minisatellite |
title_full |
Formation of pearl-necklace monomorphic G-quadruplexes in the human CEB25 minisatellite |
title_fullStr |
Formation of pearl-necklace monomorphic G-quadruplexes in the human CEB25 minisatellite |
title_full_unstemmed |
Formation of pearl-necklace monomorphic G-quadruplexes in the human CEB25 minisatellite |
title_sort |
formation of pearl-necklace monomorphic g-quadruplexes in the human ceb25 minisatellite |
publishDate |
2013 |
url |
https://hdl.handle.net/10356/95807 http://hdl.handle.net/10220/11207 |
_version_ |
1681035455180570624 |