Genome-wide gene expression profiling of the Angelman syndrome mice with Ube3a mutation
Angelman syndrome (AS) is a human neurological disorder caused by lack of maternal UBE3A expression in the brain. Currently, the pathogenesis of AS, and how deficiency of maternal UBE3A can upset cellular homeostasis, remains vague. In this study, we performed a genomewide microarray analysis on the...
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sg-ntu-dr.10356-962802022-02-16T16:28:02Z Genome-wide gene expression profiling of the Angelman syndrome mice with Ube3a mutation Low, Daren. Chen, Ken-Shiung. School of Biological Sciences DRNTU::Science::Biological sciences::Genetics Angelman syndrome (AS) is a human neurological disorder caused by lack of maternal UBE3A expression in the brain. Currently, the pathogenesis of AS, and how deficiency of maternal UBE3A can upset cellular homeostasis, remains vague. In this study, we performed a genomewide microarray analysis on the Ube3a maternal deficient (Ube3am-/p+) AS mouse to search for genes affected in the absence of Ube3a. We observed 64 differentially expressed transcripts (7 up-regulated and 57 down-regulated) showing more than 1.5-fold differences in expression (p<0.05) when compared to wildtype mice. Pathway analysis shows that these genes are implicated in three major networks associated with signalin, nervous system development and cell death. Using quantitative reverse transcription PCR (qRT-PCR), we validated the differential expression of genes (Fgf7, Glra1, Mc1r, Nr4a2, Slc5a7 and Epha6) that shows functional relevance to AS phenotype. We also show that the protein level of Mc1r and Nr4a2 in the AS mice cerebellum are decreased relative to that of the wildtype mice. Consistent with this finding, expression of small interfering RNA that targets Ube3a in P19 cells caused down-regulation of Mc1r and Nr4a2, whereas overexpression of Ube3a results in the up-regulation of Mc1r and Nr4a2 when compared to mock transfected cells. These observation aid in providing insights into the genesis of neurodevelopmental phenotype of AS and highlight specific area for future research. 2011-10-06T07:15:56Z 2019-12-06T19:28:08Z 2011-10-06T07:15:56Z 2019-12-06T19:28:08Z 2010 2010 Journal Article Low, D., & Chen, K.-S. (2010). Genome-wide gene expression profiling of the Angelman syndrome mice with Ube3a mutation. European Journal of Human Genetics, 18, 1228-1235. 1018-4813 https://hdl.handle.net/10356/96280 http://hdl.handle.net/10220/7170 10.1038/ejhg.2010.95 20571502 en European journal of human genetics © 2010 Macmillan Publishers Limited 7 p. |
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DRNTU::Science::Biological sciences::Genetics Low, Daren. Chen, Ken-Shiung. Genome-wide gene expression profiling of the Angelman syndrome mice with Ube3a mutation |
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Angelman syndrome (AS) is a human neurological disorder caused by lack of maternal UBE3A expression in the brain. Currently, the pathogenesis of AS, and how deficiency of maternal UBE3A can upset cellular homeostasis, remains vague. In this study, we performed a genomewide microarray analysis on the Ube3a maternal deficient (Ube3am-/p+) AS mouse to search for genes affected in the absence of Ube3a. We observed 64 differentially expressed transcripts (7 up-regulated and 57 down-regulated) showing more than 1.5-fold differences in expression (p<0.05) when compared to wildtype mice. Pathway analysis shows that these genes are implicated in three major networks associated with signalin, nervous system development and cell death. Using quantitative reverse transcription PCR (qRT-PCR), we validated the differential expression of genes (Fgf7, Glra1, Mc1r, Nr4a2, Slc5a7 and Epha6) that shows functional
relevance to AS phenotype. We also show that the protein level of Mc1r and Nr4a2 in the AS mice cerebellum are decreased relative to that of the wildtype mice. Consistent with this finding,
expression of small interfering RNA that targets Ube3a in P19 cells caused down-regulation of Mc1r and Nr4a2, whereas overexpression of Ube3a results in the up-regulation of Mc1r and
Nr4a2 when compared to mock transfected cells. These observation aid in providing insights into the genesis of neurodevelopmental phenotype of AS and highlight specific area for future research. |
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School of Biological Sciences |
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School of Biological Sciences Low, Daren. Chen, Ken-Shiung. |
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Article |
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Low, Daren. Chen, Ken-Shiung. |
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Low, Daren. |
title |
Genome-wide gene expression profiling of the Angelman syndrome mice with Ube3a mutation |
title_short |
Genome-wide gene expression profiling of the Angelman syndrome mice with Ube3a mutation |
title_full |
Genome-wide gene expression profiling of the Angelman syndrome mice with Ube3a mutation |
title_fullStr |
Genome-wide gene expression profiling of the Angelman syndrome mice with Ube3a mutation |
title_full_unstemmed |
Genome-wide gene expression profiling of the Angelman syndrome mice with Ube3a mutation |
title_sort |
genome-wide gene expression profiling of the angelman syndrome mice with ube3a mutation |
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2011 |
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https://hdl.handle.net/10356/96280 http://hdl.handle.net/10220/7170 |
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