Engineering of interlocked DNA G-quadruplexes as a robust scaffold

Engineering of interlocked DNA G-quadruplexes as a robust scaffold

Interlock is a structural element in DNA G-quadruplexes that can be compared with the commonly used complementary binding of ‘sticky ends’ in DNA duplexes. G-quadruplex interlocking can be a basis for the assembly of higher-order structures. In this study, we formulated a rule to engineer (3+1...

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Bibliographic Details
Main Authors: Phan, Anh Tuân, Do, Ngoc Quang
Other Authors: School of Physical and Mathematical Sciences
Format: Article
Language:English
Published: 2013
Subjects:
DRNTU::Science::Medicine::Biomedical engineering
Online Access:https://hdl.handle.net/10356/97175
http://hdl.handle.net/10220/9982
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Institution: Nanyang Technological University
Language: English
Description
Summary:Interlock is a structural element in DNA G-quadruplexes that can be compared with the commonly used complementary binding of ‘sticky ends’ in DNA duplexes. G-quadruplex interlocking can be a basis for the assembly of higher-order structures. In this study, we formulated a rule to engineer (3+1) interlocked dimeric G-quadruplexes and established the folding topology of the designed DNA sequences by nuclear magnetic resonance spectroscopy. These interlocked G-quadruplexes are very stable and can serve as compact robust scaffolds for various applications. Different structural elements can be engineered in these robust scaffolds. We demonstrated the anti-HIV inhibition activity of the newly designed DNA sequences.

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