Engineering of interlocked DNA G-quadruplexes as a robust scaffold
Interlock is a structural element in DNA G-quadruplexes that can be compared with the commonly used complementary binding of ‘sticky ends’ in DNA duplexes. G-quadruplex interlocking can be a basis for the assembly of higher-order structures. In this study, we formulated a rule to engineer (3+1...
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sg-ntu-dr.10356-971752023-02-28T16:56:26Z Engineering of interlocked DNA G-quadruplexes as a robust scaffold Phan, Anh Tuân Do, Ngoc Quang School of Physical and Mathematical Sciences DRNTU::Science::Medicine::Biomedical engineering Interlock is a structural element in DNA G-quadruplexes that can be compared with the commonly used complementary binding of ‘sticky ends’ in DNA duplexes. G-quadruplex interlocking can be a basis for the assembly of higher-order structures. In this study, we formulated a rule to engineer (3+1) interlocked dimeric G-quadruplexes and established the folding topology of the designed DNA sequences by nuclear magnetic resonance spectroscopy. These interlocked G-quadruplexes are very stable and can serve as compact robust scaffolds for various applications. Different structural elements can be engineered in these robust scaffolds. We demonstrated the anti-HIV inhibition activity of the newly designed DNA sequences. Published version 2013-05-23T07:40:34Z 2019-12-06T19:39:48Z 2013-05-23T07:40:34Z 2019-12-06T19:39:48Z 2012 2012 Journal Article Phan, A. T., & Do, N. Q. (2013). Engineering of interlocked DNA G-quadruplexes as a robust scaffold. Nucleic Acids Research, 41(4), 2683-2688. https://hdl.handle.net/10356/97175 http://hdl.handle.net/10220/9982 10.1093/nar/gks1304 23275539 en Nucleic acids research © 2012 The Author(s). This paper was published in Nucleic Acids Research and is made available as an electronic reprint (preprint) with permission of The Author(s). The paper can be found at the following official DOI: [http://dx.doi.org/10.1093/nar/gks1304]. One print or electronic copy may be made for personal use only. Systematic or multiple reproduction, distribution to multiple locations via electronic or other means, duplication of any material in this paper for a fee or for commercial purposes, or modification of the content of the paper is prohibited and is subject to penalties under law. application/pdf |
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DRNTU::Science::Medicine::Biomedical engineering Phan, Anh Tuân Do, Ngoc Quang Engineering of interlocked DNA G-quadruplexes as a robust scaffold |
description |
Interlock is a structural element in DNA
G-quadruplexes that can be compared with the commonly
used complementary binding of ‘sticky ends’
in DNA duplexes. G-quadruplex interlocking can be
a basis for the assembly of higher-order structures.
In this study, we formulated a rule to engineer
(3+1) interlocked dimeric G-quadruplexes and established
the folding topology of the designed DNA
sequences by nuclear magnetic resonance spectroscopy.
These interlocked G-quadruplexes are very stable and can serve as compact robust scaffolds for various applications. Different structural elements can be engineered in these robust scaffolds. We demonstrated the anti-HIV inhibition
activity of the newly designed DNA sequences. |
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School of Physical and Mathematical Sciences |
author_facet |
School of Physical and Mathematical Sciences Phan, Anh Tuân Do, Ngoc Quang |
format |
Article |
author |
Phan, Anh Tuân Do, Ngoc Quang |
author_sort |
Phan, Anh Tuân |
title |
Engineering of interlocked DNA G-quadruplexes as a robust scaffold |
title_short |
Engineering of interlocked DNA G-quadruplexes as a robust scaffold |
title_full |
Engineering of interlocked DNA G-quadruplexes as a robust scaffold |
title_fullStr |
Engineering of interlocked DNA G-quadruplexes as a robust scaffold |
title_full_unstemmed |
Engineering of interlocked DNA G-quadruplexes as a robust scaffold |
title_sort |
engineering of interlocked dna g-quadruplexes as a robust scaffold |
publishDate |
2013 |
url |
https://hdl.handle.net/10356/97175 http://hdl.handle.net/10220/9982 |
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1759856557265256448 |