Engineering selection stringency on expression vector for the production of recombinant human alpha1-antitrypsin using Chinese Hamster ovary cells

• Main Authors: Chin, C.L, Chin, H.K, Chin, C, Lai, E.T, Ng, S.K
• Other Authors: PHARMACY
• Format: Article
• Published: 2020
• Subjects: Amino acids; Animal cell culture; Batch cell culture; Body fluids; Cell culture; Cells; Cytology; Enzyme activity; Gene expression; Gene expression regulation; Genes; Interferons; Lakes; Macromolecules; Mammals; Multiobjective optimization; Productivity; Proteins; Recombinant proteins; Site selection; Transcription; Translation (languages); Vectors; Alpha 1-antitrypsin; De-optimization; Dihydrofolate reductase; Expression vectors; Internal ribosome entry site; Selection stringency; Cell engineering; alpha 1 antitrypsin; dihydrofolate reductase; molecular marker; recombinant alpha 1 antitrypsin; unclassified drug; biological marker; internal ribosome entry site; methotrexate; ornithine decarboxylase; recombinant protein; animal cell; Article; cell culture; cell survival; Chinese hamster; CHO cell line; codon deoptimization; codon usage; concentration response; controlled study; dhfr gene; expression vector; female; gene amplification; gene dosage; gene expression; genetic linkage; genetic selection; human; molecular weight; nonhuman; point mutation; process optimization; protein degradation; protein engineering; protein motif; protein synthesis; rhA1AT gene; tandem PEST sequence; tandem repeat; translation initiation; animal; Cricetulus; gene vector; genetics; metabolism; mouse; mutation; procedures; Cricetulus griseus; Mammalia; Murinae; alpha 1-Antitrypsin; Animals; Biomarkers; CHO Cells; Gene Amplification; Genetic Vectors; Humans; Internal Ribosome Entry Sites; Methotrexate; Mice; Mutation; Ornithine Decarboxylase; Protein Engineering; Recombinant Proteins; Tetrahydrofolate Dehydrogenase
• Online Access: https://scholarbank.nus.edu.sg/handle/10635/181448
• Institution: National University of Singapore

10.1186/s12896-015-0145-9