Engineering selection stringency on expression vector for the production of recombinant human alpha1-antitrypsin using Chinese Hamster ovary cells

Engineering selection stringency on expression vector for the production of recombinant human alpha1-antitrypsin using Chinese Hamster ovary cells

10.1186/s12896-015-0145-9

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Bibliographic Details
Main Authors:	Chin, C.L, Chin, H.K, Chin, C, Lai, E.T, Ng, S.K
Other Authors:	PHARMACY
Format:	Article
Published:	2020
Subjects:	Amino acids Animal cell culture Batch cell culture Body fluids Cell culture Cells Cytology Enzyme activity Gene expression Gene expression regulation Genes Interferons Lakes Macromolecules Mammals Multiobjective optimization Productivity Proteins Recombinant proteins Site selection Transcription Translation (languages) Vectors Alpha 1-antitrypsin De-optimization Dihydrofolate reductase Expression vectors Internal ribosome entry site Selection stringency Cell engineering alpha 1 antitrypsin dihydrofolate reductase molecular marker recombinant alpha 1 antitrypsin unclassified drug biological marker internal ribosome entry site methotrexate ornithine decarboxylase recombinant protein animal cell Article cell culture cell survival Chinese hamster CHO cell line codon deoptimization codon usage concentration response controlled study dhfr gene expression vector female gene amplification gene dosage gene expression genetic linkage genetic selection human molecular weight nonhuman point mutation process optimization protein degradation protein engineering protein motif protein synthesis rhA1AT gene tandem PEST sequence tandem repeat translation initiation animal Cricetulus gene vector genetics metabolism mouse mutation procedures Cricetulus griseus Mammalia Murinae alpha 1-Antitrypsin Animals Biomarkers CHO Cells Gene Amplification Genetic Vectors Humans Internal Ribosome Entry Sites Methotrexate Mice Mutation Ornithine Decarboxylase Protein Engineering Recombinant Proteins Tetrahydrofolate Dehydrogenase
Online Access:	https://scholarbank.nus.edu.sg/handle/10635/181448
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Institution:	National University of Singapore

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