Engineering selection stringency on expression vector for the production of recombinant human alpha1-antitrypsin using Chinese Hamster ovary cells

10.1186/s12896-015-0145-9

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Main Authors:	Chin, C.L, Chin, H.K, Chin, C, Lai, E.T, Ng, S.K
Other Authors:	PHARMACY
Format:	Article
Published:	2020
Subjects:	Amino acids Animal cell culture Batch cell culture Body fluids Cell culture Cells Cytology Enzyme activity Gene expression Gene expression regulation Genes Interferons Lakes Macromolecules Mammals Multiobjective optimization Productivity Proteins Recombinant proteins Site selection Transcription Translation (languages) Vectors Alpha 1-antitrypsin De-optimization Dihydrofolate reductase Expression vectors Internal ribosome entry site Selection stringency Cell engineering alpha 1 antitrypsin dihydrofolate reductase molecular marker recombinant alpha 1 antitrypsin unclassified drug biological marker internal ribosome entry site methotrexate ornithine decarboxylase recombinant protein animal cell Article cell culture cell survival Chinese hamster CHO cell line codon deoptimization codon usage concentration response controlled study dhfr gene expression vector female gene amplification gene dosage gene expression genetic linkage genetic selection human molecular weight nonhuman point mutation process optimization protein degradation protein engineering protein motif protein synthesis rhA1AT gene tandem PEST sequence tandem repeat translation initiation animal Cricetulus gene vector genetics metabolism mouse mutation procedures Cricetulus griseus Mammalia Murinae alpha 1-Antitrypsin Animals Biomarkers CHO Cells Gene Amplification Genetic Vectors Humans Internal Ribosome Entry Sites Methotrexate Mice Mutation Ornithine Decarboxylase Protein Engineering Recombinant Proteins Tetrahydrofolate Dehydrogenase
Online Access:	https://scholarbank.nus.edu.sg/handle/10635/181448
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Institution:	National University of Singapore

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spelling	sg-nus-scholar.10635-1814482024-04-05T09:03:57Z Engineering selection stringency on expression vector for the production of recombinant human alpha1-antitrypsin using Chinese Hamster ovary cells Chin, C.L Chin, H.K Chin, C Lai, E.T Ng, S.K PHARMACY Amino acids Animal cell culture Batch cell culture Body fluids Cell culture Cells Cytology Enzyme activity Gene expression Gene expression regulation Genes Interferons Lakes Macromolecules Mammals Multiobjective optimization Productivity Proteins Recombinant proteins Site selection Transcription Translation (languages) Vectors Alpha 1-antitrypsin De-optimization Dihydrofolate reductase Expression vectors Internal ribosome entry site Selection stringency Cell engineering alpha 1 antitrypsin dihydrofolate reductase molecular marker recombinant alpha 1 antitrypsin unclassified drug alpha 1 antitrypsin biological marker dihydrofolate reductase internal ribosome entry site methotrexate ornithine decarboxylase recombinant protein animal cell Article cell culture cell survival Chinese hamster CHO cell line codon deoptimization codon usage concentration response controlled study dhfr gene expression vector female gene amplification gene dosage gene expression genetic linkage genetic selection human internal ribosome entry site molecular weight nonhuman point mutation process optimization protein degradation protein engineering protein motif protein synthesis rhA1AT gene tandem PEST sequence tandem repeat translation initiation animal CHO cell line Cricetulus gene vector genetics internal ribosome entry site metabolism mouse mutation procedures protein engineering Cricetulus griseus Mammalia Murinae alpha 1-Antitrypsin Animals Biomarkers CHO Cells Cricetulus Gene Amplification Genetic Vectors Humans Internal Ribosome Entry Sites Methotrexate Mice Mutation Ornithine Decarboxylase Protein Engineering Recombinant Proteins Tetrahydrofolate Dehydrogenase 10.1186/s12896-015-0145-9 BMC Biotechnology 15 1 44 2020-10-27T10:57:37Z 2020-10-27T10:57:37Z 2015 Article Chin, C.L, Chin, H.K, Chin, C, Lai, E.T, Ng, S.K (2015). Engineering selection stringency on expression vector for the production of recombinant human alpha1-antitrypsin using Chinese Hamster ovary cells. BMC Biotechnology 15 (1) : 44. ScholarBank@NUS Repository. https://doi.org/10.1186/s12896-015-0145-9 14726750 https://scholarbank.nus.edu.sg/handle/10635/181448 Attribution 4.0 International http://creativecommons.org/licenses/by/4.0/ Unpaywall 20201031
institution	National University of Singapore
building	NUS Library
continent	Asia
country	Singapore Singapore
content_provider	NUS Library
collection	ScholarBank@NUS
topic	Amino acids Animal cell culture Batch cell culture Body fluids Cell culture Cells Cytology Enzyme activity Gene expression Gene expression regulation Genes Interferons Lakes Macromolecules Mammals Multiobjective optimization Productivity Proteins Recombinant proteins Site selection Transcription Translation (languages) Vectors Alpha 1-antitrypsin De-optimization Dihydrofolate reductase Expression vectors Internal ribosome entry site Selection stringency Cell engineering alpha 1 antitrypsin dihydrofolate reductase molecular marker recombinant alpha 1 antitrypsin unclassified drug alpha 1 antitrypsin biological marker dihydrofolate reductase internal ribosome entry site methotrexate ornithine decarboxylase recombinant protein animal cell Article cell culture cell survival Chinese hamster CHO cell line codon deoptimization codon usage concentration response controlled study dhfr gene expression vector female gene amplification gene dosage gene expression genetic linkage genetic selection human internal ribosome entry site molecular weight nonhuman point mutation process optimization protein degradation protein engineering protein motif protein synthesis rhA1AT gene tandem PEST sequence tandem repeat translation initiation animal CHO cell line Cricetulus gene vector genetics internal ribosome entry site metabolism mouse mutation procedures protein engineering Cricetulus griseus Mammalia Murinae alpha 1-Antitrypsin Animals Biomarkers CHO Cells Cricetulus Gene Amplification Genetic Vectors Humans Internal Ribosome Entry Sites Methotrexate Mice Mutation Ornithine Decarboxylase Protein Engineering Recombinant Proteins Tetrahydrofolate Dehydrogenase
spellingShingle	Amino acids Animal cell culture Batch cell culture Body fluids Cell culture Cells Cytology Enzyme activity Gene expression Gene expression regulation Genes Interferons Lakes Macromolecules Mammals Multiobjective optimization Productivity Proteins Recombinant proteins Site selection Transcription Translation (languages) Vectors Alpha 1-antitrypsin De-optimization Dihydrofolate reductase Expression vectors Internal ribosome entry site Selection stringency Cell engineering alpha 1 antitrypsin dihydrofolate reductase molecular marker recombinant alpha 1 antitrypsin unclassified drug alpha 1 antitrypsin biological marker dihydrofolate reductase internal ribosome entry site methotrexate ornithine decarboxylase recombinant protein animal cell Article cell culture cell survival Chinese hamster CHO cell line codon deoptimization codon usage concentration response controlled study dhfr gene expression vector female gene amplification gene dosage gene expression genetic linkage genetic selection human internal ribosome entry site molecular weight nonhuman point mutation process optimization protein degradation protein engineering protein motif protein synthesis rhA1AT gene tandem PEST sequence tandem repeat translation initiation animal CHO cell line Cricetulus gene vector genetics internal ribosome entry site metabolism mouse mutation procedures protein engineering Cricetulus griseus Mammalia Murinae alpha 1-Antitrypsin Animals Biomarkers CHO Cells Cricetulus Gene Amplification Genetic Vectors Humans Internal Ribosome Entry Sites Methotrexate Mice Mutation Ornithine Decarboxylase Protein Engineering Recombinant Proteins Tetrahydrofolate Dehydrogenase Chin, C.L Chin, H.K Chin, C Lai, E.T Ng, S.K Engineering selection stringency on expression vector for the production of recombinant human alpha1-antitrypsin using Chinese Hamster ovary cells
description	10.1186/s12896-015-0145-9
author2	PHARMACY
author_facet	PHARMACY Chin, C.L Chin, H.K Chin, C Lai, E.T Ng, S.K
format	Article
author	Chin, C.L Chin, H.K Chin, C Lai, E.T Ng, S.K
author_sort	Chin, C.L
title	Engineering selection stringency on expression vector for the production of recombinant human alpha1-antitrypsin using Chinese Hamster ovary cells
title_short	Engineering selection stringency on expression vector for the production of recombinant human alpha1-antitrypsin using Chinese Hamster ovary cells
title_full	Engineering selection stringency on expression vector for the production of recombinant human alpha1-antitrypsin using Chinese Hamster ovary cells
title_fullStr	Engineering selection stringency on expression vector for the production of recombinant human alpha1-antitrypsin using Chinese Hamster ovary cells
title_full_unstemmed	Engineering selection stringency on expression vector for the production of recombinant human alpha1-antitrypsin using Chinese Hamster ovary cells
title_sort	engineering selection stringency on expression vector for the production of recombinant human alpha1-antitrypsin using chinese hamster ovary cells
publishDate	2020
url	https://scholarbank.nus.edu.sg/handle/10635/181448
_version_	1800914638238384128

Engineering selection stringency on expression vector for the production of recombinant human alpha1-antitrypsin using Chinese Hamster ovary cells

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