Production of monoclonal antibodies to P-glycoprotein: Its application in detection of soluble and surface P-glycoprotein of leukemia patients

• Main Authors: Chiampanichayakul S., Anuchapreeda S., Chruewkamlow N., Mahasongkram K., Thanaratanakorn P., Kasinrerk W.
• Format: Article
• Language: English
• Published: 2014
• Online Access: http://www.scopus.com/inward/record.url?eid=2-s2.0-77956896273&partnerID=40&md5=7a0918b59903cbbded5b676b0e358f15; http://www.ncbi.nlm.nih.gov/pubmed/20740331; http://cmuir.cmu.ac.th/handle/6653943832/2545
• Institution: Chiang Mai University

Multidrug resistance (MDR) in leukemia is commonly associated with the expression of a transmembrane protein, P-glycoprotein (P-gp). In this study, two monoclonal antibodies (mAbs) specific for the extracellular domain of P-gp were generated. By employing the generated mAbs, a two-color lysed whole blood flow cytometric method for surface P-gp and an efficient sandwich ELISA for soluble P-gp determinations were established. By using the established methods, surface and soluble P-gp were detected in several leukemia patients. The presence of soluble P-gp could be used to identify the P-gp surface expression patients. Detection of soluble P-gp reported provides a new basis that may lead to a better understanding of the MDR mechanism in leukemia. © 2010 The Japanese Society of Hematology.