Reversal of cardiac iron loading and dysfunction in thalassemic mice by curcuminoids

Non-transferrin bound iron (NTBI) is found in plasma of β-thalassemia patients and causes oxidative tissue damage. Cardiac siderosis and complications are the secondary cause of death in β-thalassemia major patients. Desferrioxamine (DFO), deferiprone (DFP) and deferasirox (DFX) are promising chelat...

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Main Authors: Thephinlap C., Phisalaphong C., Lailerd N., Chattipakorn N., Winichagoon P., Vadolus J., Fucharoen S., Porter J.B., Srichairatanakool S.
Format: Article
Language:English
Published: 2014
Online Access:http://www.scopus.com/inward/record.url?eid=2-s2.0-78751479300&partnerID=40&md5=784a8d5e002348796d4084e9c4dc0243
http://www.ncbi.nlm.nih.gov/pubmed/21235521
http://cmuir.cmu.ac.th/handle/6653943832/2723
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spelling th-cmuir.6653943832-27232014-08-30T02:25:19Z Reversal of cardiac iron loading and dysfunction in thalassemic mice by curcuminoids Thephinlap C. Phisalaphong C. Lailerd N. Chattipakorn N. Winichagoon P. Vadolus J. Fucharoen S. Porter J.B. Srichairatanakool S. Non-transferrin bound iron (NTBI) is found in plasma of β-thalassemia patients and causes oxidative tissue damage. Cardiac siderosis and complications are the secondary cause of death in β-thalassemia major patients. Desferrioxamine (DFO), deferiprone (DFP) and deferasirox (DFX) are promising chelators used to get negative iron balance and improve life quality. DFP has been shown to remove myocardial iron effectively. Curcuminoids (CUR) can chelate plasma NTBI, inhibit lipid peroxidation and alleviate cardiac autonomic imbalance. Effects of CUR on cardiac iron deposition and function were investigated in iron-loaded mice. Wild type (muβ+/+; WT) and heterozygous β-knockout (muβth-3/+; BKO) mice (C57BL/6) were fed with ferrocene-supplemented diet (Fe diet) and coincidently intervened with CUR and DFP for 2 months. Concentrations of plasma NTBI and malondialdehyde (MDA) were measured using HPLC techniques. Heart iron concentration was determined based on atomic absorption spectrophotometry and Perl's staining methods. Short-term electrocardiogram (ECG) was recorded with AD Instruments Power Lab, and heart rate variability (HRV) was evaluated using MATLAB 7.0 program. Fe diet increased levels of NTBI and MDA in plasma, nonheme iron and iron deposit in heart tissue significantly, and depressed the HRV, which the levels were higher in the BKO mice than the WT mice. CUR and DFP treatments lowered plasma NTBI as well as MDA concentrations (p <0.05), heart iron accumulation effectively, and also improved the HRV in the treated mice. The results imply that CUR would be effective in decreasing plasma NTBI and myocardial iron, alleviating lipid peroxidation and improving cardiac function in iron-loaded thalassemic mice. © 2011 Bentham Science Publishers Ltd. 2014-08-30T02:25:19Z 2014-08-30T02:25:19Z 2011 Article 15734064 10.2174/157340611794072724 21235521 http://www.scopus.com/inward/record.url?eid=2-s2.0-78751479300&partnerID=40&md5=784a8d5e002348796d4084e9c4dc0243 http://www.ncbi.nlm.nih.gov/pubmed/21235521 http://cmuir.cmu.ac.th/handle/6653943832/2723 English
institution Chiang Mai University
building Chiang Mai University Library
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language English
description Non-transferrin bound iron (NTBI) is found in plasma of β-thalassemia patients and causes oxidative tissue damage. Cardiac siderosis and complications are the secondary cause of death in β-thalassemia major patients. Desferrioxamine (DFO), deferiprone (DFP) and deferasirox (DFX) are promising chelators used to get negative iron balance and improve life quality. DFP has been shown to remove myocardial iron effectively. Curcuminoids (CUR) can chelate plasma NTBI, inhibit lipid peroxidation and alleviate cardiac autonomic imbalance. Effects of CUR on cardiac iron deposition and function were investigated in iron-loaded mice. Wild type (muβ+/+; WT) and heterozygous β-knockout (muβth-3/+; BKO) mice (C57BL/6) were fed with ferrocene-supplemented diet (Fe diet) and coincidently intervened with CUR and DFP for 2 months. Concentrations of plasma NTBI and malondialdehyde (MDA) were measured using HPLC techniques. Heart iron concentration was determined based on atomic absorption spectrophotometry and Perl's staining methods. Short-term electrocardiogram (ECG) was recorded with AD Instruments Power Lab, and heart rate variability (HRV) was evaluated using MATLAB 7.0 program. Fe diet increased levels of NTBI and MDA in plasma, nonheme iron and iron deposit in heart tissue significantly, and depressed the HRV, which the levels were higher in the BKO mice than the WT mice. CUR and DFP treatments lowered plasma NTBI as well as MDA concentrations (p <0.05), heart iron accumulation effectively, and also improved the HRV in the treated mice. The results imply that CUR would be effective in decreasing plasma NTBI and myocardial iron, alleviating lipid peroxidation and improving cardiac function in iron-loaded thalassemic mice. © 2011 Bentham Science Publishers Ltd.
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author Thephinlap C.
Phisalaphong C.
Lailerd N.
Chattipakorn N.
Winichagoon P.
Vadolus J.
Fucharoen S.
Porter J.B.
Srichairatanakool S.
spellingShingle Thephinlap C.
Phisalaphong C.
Lailerd N.
Chattipakorn N.
Winichagoon P.
Vadolus J.
Fucharoen S.
Porter J.B.
Srichairatanakool S.
Reversal of cardiac iron loading and dysfunction in thalassemic mice by curcuminoids
author_facet Thephinlap C.
Phisalaphong C.
Lailerd N.
Chattipakorn N.
Winichagoon P.
Vadolus J.
Fucharoen S.
Porter J.B.
Srichairatanakool S.
author_sort Thephinlap C.
title Reversal of cardiac iron loading and dysfunction in thalassemic mice by curcuminoids
title_short Reversal of cardiac iron loading and dysfunction in thalassemic mice by curcuminoids
title_full Reversal of cardiac iron loading and dysfunction in thalassemic mice by curcuminoids
title_fullStr Reversal of cardiac iron loading and dysfunction in thalassemic mice by curcuminoids
title_full_unstemmed Reversal of cardiac iron loading and dysfunction in thalassemic mice by curcuminoids
title_sort reversal of cardiac iron loading and dysfunction in thalassemic mice by curcuminoids
publishDate 2014
url http://www.scopus.com/inward/record.url?eid=2-s2.0-78751479300&partnerID=40&md5=784a8d5e002348796d4084e9c4dc0243
http://www.ncbi.nlm.nih.gov/pubmed/21235521
http://cmuir.cmu.ac.th/handle/6653943832/2723
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