Characterization and comparative study of polyphenol oxidases from four cultivars of Thai Solanum melogena fruits

• Main Authors: Chanasut U., Rattanapanone N.
• Format: Article
• Published: Chiang Mai University 2015
• Subjects: Multidisciplinary
• Online Access: http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=84872407838&origin=inward; http://cmuir.cmu.ac.th/handle/6653943832/38673
• Institution: Chiang Mai University

This study characterized the polyphenol oxidases (PPO) from four cultivars of eggplant (Thai Solanum melogena), representing a variety of fruit characteristics, textures, and peel colors. The study compared the effects of different substrate specificity, temperature, pH, thermal stability, and inhibitors on PPO activity to determine the optimal conditions for generating natural browning agents. Khaow yoa (OW) and Moung khan khew (OP) cultivars had the highest specific activity (OW: 631.33 ± 38.29 ΔOD min-1 mgProtein-1; OP: 652.54 ± 9.59 ΔOD min-1mgProtein-1), using 4-methylcatechol as a substrate. The best substrate for PPO from each cultivar was different: catechin for cultivars OW and OP, 4-MC for Choa pra ya (RG), and 4-tert butylcatechol for Khaew yoa (OG). Using 4-methylcatechol as a substrate, the optimal conditions for maximizing PPO activity were similar for all four cultivars: a pH of 6.0, a temperature of 30oC, and an enzyme concentration of 1% (v/v). For all cultivars, PPO activity decreased with increasing inactivation time after the temperature reached 30oC. The study tested the inhibitory effects of compounds such as ascorbic acid, citric acid, sodium chloride, sodium metabisulfite, and EDTA on the activity of residual enzyme. Ascorbic acid and sodium metabisulfite were the most effective inhibitors for these eggplant PPOs.