Influences of buffer systems on enzymatic saccharification of rice husk holocellulose and fermenting ability of various ethanol producing microorganisms

© 2017, Chiang Mai University. All rights reserved. Four saccharification buffers, including acetate, citrate, phthalate and succinate buffers, were evaluated for their effects on both saccharification and fermentation of rice husk holocellulose. At a concentration of 30 mM, all buffer systems were...

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Main Authors: Woottichai Nachaiwieng, Apinun Kanpiengjai, Takashi Watanabe, Chartchai Khanongnuch
Format: Journal
Published: 2018
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http://cmuir.cmu.ac.th/jspui/handle/6653943832/46413
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spelling th-cmuir.6653943832-464132018-04-25T07:25:56Z Influences of buffer systems on enzymatic saccharification of rice husk holocellulose and fermenting ability of various ethanol producing microorganisms Woottichai Nachaiwieng Apinun Kanpiengjai Takashi Watanabe Chartchai Khanongnuch Chemistry Materials Science Mathematics Agricultural and Biological Sciences © 2017, Chiang Mai University. All rights reserved. Four saccharification buffers, including acetate, citrate, phthalate and succinate buffers, were evaluated for their effects on both saccharification and fermentation of rice husk holocellulose. At a concentration of 30 mM, all buffer systems were found to be suitable for saccharification by three commercial cellulolytic enzymes (Meicelase, Accellerase 1500 and Accellerase XC). However, at concentrations of 50-200 mM, citrate and acetate buffers partially inhibited the fermenting ability of Saccharomyces cerevisiase, Zymomonas mobilis, Kluyveromyces marxianus, Candida shehatae and Pichia stipitis. This was in contrast to phthalate and succinate buffers in which these organisms retained 100% relative fermenting ability within concentrations in the range of 50-500 mM. This is the first report indicating potential buffers appropriate for broad ethanol producing microorganisms in bioethanol fermentation, particularly for co-culture fermentation of cellulosic hydrolysate. 2018-04-25T06:54:32Z 2018-04-25T06:54:32Z 2017-04-01 Journal 01252526 2-s2.0-85018492364 https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85018492364&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/46413
institution Chiang Mai University
building Chiang Mai University Library
country Thailand
collection CMU Intellectual Repository
topic Chemistry
Materials Science
Mathematics
Agricultural and Biological Sciences
spellingShingle Chemistry
Materials Science
Mathematics
Agricultural and Biological Sciences
Woottichai Nachaiwieng
Apinun Kanpiengjai
Takashi Watanabe
Chartchai Khanongnuch
Influences of buffer systems on enzymatic saccharification of rice husk holocellulose and fermenting ability of various ethanol producing microorganisms
description © 2017, Chiang Mai University. All rights reserved. Four saccharification buffers, including acetate, citrate, phthalate and succinate buffers, were evaluated for their effects on both saccharification and fermentation of rice husk holocellulose. At a concentration of 30 mM, all buffer systems were found to be suitable for saccharification by three commercial cellulolytic enzymes (Meicelase, Accellerase 1500 and Accellerase XC). However, at concentrations of 50-200 mM, citrate and acetate buffers partially inhibited the fermenting ability of Saccharomyces cerevisiase, Zymomonas mobilis, Kluyveromyces marxianus, Candida shehatae and Pichia stipitis. This was in contrast to phthalate and succinate buffers in which these organisms retained 100% relative fermenting ability within concentrations in the range of 50-500 mM. This is the first report indicating potential buffers appropriate for broad ethanol producing microorganisms in bioethanol fermentation, particularly for co-culture fermentation of cellulosic hydrolysate.
format Journal
author Woottichai Nachaiwieng
Apinun Kanpiengjai
Takashi Watanabe
Chartchai Khanongnuch
author_facet Woottichai Nachaiwieng
Apinun Kanpiengjai
Takashi Watanabe
Chartchai Khanongnuch
author_sort Woottichai Nachaiwieng
title Influences of buffer systems on enzymatic saccharification of rice husk holocellulose and fermenting ability of various ethanol producing microorganisms
title_short Influences of buffer systems on enzymatic saccharification of rice husk holocellulose and fermenting ability of various ethanol producing microorganisms
title_full Influences of buffer systems on enzymatic saccharification of rice husk holocellulose and fermenting ability of various ethanol producing microorganisms
title_fullStr Influences of buffer systems on enzymatic saccharification of rice husk holocellulose and fermenting ability of various ethanol producing microorganisms
title_full_unstemmed Influences of buffer systems on enzymatic saccharification of rice husk holocellulose and fermenting ability of various ethanol producing microorganisms
title_sort influences of buffer systems on enzymatic saccharification of rice husk holocellulose and fermenting ability of various ethanol producing microorganisms
publishDate 2018
url https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85018492364&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/46413
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