Characterization of γ-glutamyl hydrolase produced by Bacillus sp. isolated from Thai Thua-nao
γ-Glutamyl hydrolase with a molecular mass of 28 kDa was purified from the culture broth of Bacillus sp. isolated from Thai Thua-nao, a natto-like fermented soybean food. The purified enzyme hydrolyzed chemically synthesized oligo-γ-L-glutamates but not oligo-γ-D-glutamates and degraded γ-polyglutam...
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2014
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th-cmuir.6653943832-50482014-08-30T02:56:06Z Characterization of γ-glutamyl hydrolase produced by Bacillus sp. isolated from Thai Thua-nao Chunhachart O. Itoh T. Sukchotiratana M. Tanimoto H. Tahara Y. γ-Glutamyl hydrolase with a molecular mass of 28 kDa was purified from the culture broth of Bacillus sp. isolated from Thai Thua-nao, a natto-like fermented soybean food. The purified enzyme hydrolyzed chemically synthesized oligo-γ-L-glutamates but not oligo-γ-D-glutamates and degraded γ-polyglutamic acid to a hydrolyzed product of only about 20 kDa (with D- and L-glutamic acid in a ratio of 70:30), suggesting that the enzyme is a γ-glutamyl hydrolase that cleaves the γ-glutamyl linkage between L- and L-glutamic acid of γ-polyglutamic acid. 2014-08-30T02:56:06Z 2014-08-30T02:56:06Z 2006 Article 09168451 10.1271/bbb.60280 17090946 BBBIE http://www.scopus.com/inward/record.url?eid=2-s2.0-33751437461&partnerID=40&md5=f99ce021474bb4669273f30141916a68 http://cmuir.cmu.ac.th/handle/6653943832/5048 English |
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γ-Glutamyl hydrolase with a molecular mass of 28 kDa was purified from the culture broth of Bacillus sp. isolated from Thai Thua-nao, a natto-like fermented soybean food. The purified enzyme hydrolyzed chemically synthesized oligo-γ-L-glutamates but not oligo-γ-D-glutamates and degraded γ-polyglutamic acid to a hydrolyzed product of only about 20 kDa (with D- and L-glutamic acid in a ratio of 70:30), suggesting that the enzyme is a γ-glutamyl hydrolase that cleaves the γ-glutamyl linkage between L- and L-glutamic acid of γ-polyglutamic acid. |
| format |
Article |
| author |
Chunhachart O. Itoh T. Sukchotiratana M. Tanimoto H. Tahara Y. |
| spellingShingle |
Chunhachart O. Itoh T. Sukchotiratana M. Tanimoto H. Tahara Y. Characterization of γ-glutamyl hydrolase produced by Bacillus sp. isolated from Thai Thua-nao |
| author_facet |
Chunhachart O. Itoh T. Sukchotiratana M. Tanimoto H. Tahara Y. |
| author_sort |
Chunhachart O. |
| title |
Characterization of γ-glutamyl hydrolase produced by Bacillus sp. isolated from Thai Thua-nao |
| title_short |
Characterization of γ-glutamyl hydrolase produced by Bacillus sp. isolated from Thai Thua-nao |
| title_full |
Characterization of γ-glutamyl hydrolase produced by Bacillus sp. isolated from Thai Thua-nao |
| title_fullStr |
Characterization of γ-glutamyl hydrolase produced by Bacillus sp. isolated from Thai Thua-nao |
| title_full_unstemmed |
Characterization of γ-glutamyl hydrolase produced by Bacillus sp. isolated from Thai Thua-nao |
| title_sort |
characterization of γ-glutamyl hydrolase produced by bacillus sp. isolated from thai thua-nao |
| publishDate |
2014 |
| url |
http://www.scopus.com/inward/record.url?eid=2-s2.0-33751437461&partnerID=40&md5=f99ce021474bb4669273f30141916a68 http://cmuir.cmu.ac.th/handle/6653943832/5048 |
| _version_ |
1681420351848841216 |