Medium optimization for β-galactosidase production by a thermotolerant yeast

© 2015, Chiang Mai University. All rights reserved. Kluyveromyces sp. CK8 was previously isolated from rotten fruit in northern Thailand and found to be capable of growing at 45°C. This research aimed to identify the yeast isolate and to optimize its production of β-galactosidase in synthetic medium...

Full description

Saved in:
Bibliographic Details
Main Authors: Sirinda Am-Aiam, Chartchai Khanongnuch
Format: Journal
Published: 2018
Subjects:
Online Access:https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84946565698&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/54167
Tags: Add Tag
No Tags, Be the first to tag this record!
Institution: Chiang Mai University
id th-cmuir.6653943832-54167
record_format dspace
spelling th-cmuir.6653943832-541672018-09-04T10:26:14Z Medium optimization for β-galactosidase production by a thermotolerant yeast Sirinda Am-Aiam Chartchai Khanongnuch Biochemistry, Genetics and Molecular Biology Chemistry Materials Science Mathematics Physics and Astronomy © 2015, Chiang Mai University. All rights reserved. Kluyveromyces sp. CK8 was previously isolated from rotten fruit in northern Thailand and found to be capable of growing at 45°C. This research aimed to identify the yeast isolate and to optimize its production of β-galactosidase in synthetic medium using lactose as the sole carbon source. Molecular identification via phylogenetic analysis, based on comparative sequence analysis of the 26S rRNA gene at the D1/D2 domain, revealed that isolate CK8 was Kluyveromyces marxianus. The yeast isolate produced β-galactosidase 5.33 U/ml culture when cultivated at 37°C for 18 h in Yeast-Malt extract (YM) medium containing 1% (w/v) lactose as the sole carbon source. Optimization of medium composition for enzyme production was conducted using 2-Level Factorial experimental design to determine the most relevant variables of the culture medium composition. Among five parameters, yeast extract, (NH4)2SO4 and KH2PO4 were significant effectors at p<0.05. A central composite design (CCD) and response surface plot predicted the highest β-galactosidase activity of 8.94 U/ml culture in the medium containing (g/l) of the following: yeast extract (0.23), (NH4)2SO4 (9.64), KH2PO4 (7.0), lactose (20) and MgSO4.7H2O (0.1). A validation time course culture study conducted for 18 h in the optimal medium found that the actual maximum value of enzyme activity was 8.82 U/ml culture which is 98.66% of the predicted value. 2018-09-04T10:08:52Z 2018-09-04T10:08:52Z 2015-01-01 Journal 01252526 2-s2.0-84946565698 https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84946565698&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/54167
institution Chiang Mai University
building Chiang Mai University Library
country Thailand
collection CMU Intellectual Repository
topic Biochemistry, Genetics and Molecular Biology
Chemistry
Materials Science
Mathematics
Physics and Astronomy
spellingShingle Biochemistry, Genetics and Molecular Biology
Chemistry
Materials Science
Mathematics
Physics and Astronomy
Sirinda Am-Aiam
Chartchai Khanongnuch
Medium optimization for β-galactosidase production by a thermotolerant yeast
description © 2015, Chiang Mai University. All rights reserved. Kluyveromyces sp. CK8 was previously isolated from rotten fruit in northern Thailand and found to be capable of growing at 45°C. This research aimed to identify the yeast isolate and to optimize its production of β-galactosidase in synthetic medium using lactose as the sole carbon source. Molecular identification via phylogenetic analysis, based on comparative sequence analysis of the 26S rRNA gene at the D1/D2 domain, revealed that isolate CK8 was Kluyveromyces marxianus. The yeast isolate produced β-galactosidase 5.33 U/ml culture when cultivated at 37°C for 18 h in Yeast-Malt extract (YM) medium containing 1% (w/v) lactose as the sole carbon source. Optimization of medium composition for enzyme production was conducted using 2-Level Factorial experimental design to determine the most relevant variables of the culture medium composition. Among five parameters, yeast extract, (NH4)2SO4 and KH2PO4 were significant effectors at p<0.05. A central composite design (CCD) and response surface plot predicted the highest β-galactosidase activity of 8.94 U/ml culture in the medium containing (g/l) of the following: yeast extract (0.23), (NH4)2SO4 (9.64), KH2PO4 (7.0), lactose (20) and MgSO4.7H2O (0.1). A validation time course culture study conducted for 18 h in the optimal medium found that the actual maximum value of enzyme activity was 8.82 U/ml culture which is 98.66% of the predicted value.
format Journal
author Sirinda Am-Aiam
Chartchai Khanongnuch
author_facet Sirinda Am-Aiam
Chartchai Khanongnuch
author_sort Sirinda Am-Aiam
title Medium optimization for β-galactosidase production by a thermotolerant yeast
title_short Medium optimization for β-galactosidase production by a thermotolerant yeast
title_full Medium optimization for β-galactosidase production by a thermotolerant yeast
title_fullStr Medium optimization for β-galactosidase production by a thermotolerant yeast
title_full_unstemmed Medium optimization for β-galactosidase production by a thermotolerant yeast
title_sort medium optimization for β-galactosidase production by a thermotolerant yeast
publishDate 2018
url https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84946565698&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/54167
_version_ 1681424270789443584