Development and stability evaluation of vaginal suppository containing glycyrrhiza glabra L. For the treatment of Candida albicans infection

© 2018 The Authors. Objective: The present study assessed the antioxidant activity, phytochemical content, and anti-Candida albicans property of ethanolic extract of Glycyrrhiza glabra L. (licorice). In addition, suppository formula (SF) was developed with licorice extract (LE), and the stability of...

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Main Authors: Sasithorn Sirilun, Bhagavathi Sundaram Sivamaruthi, Periyanaina Kesika, Noppawat Pengkumsri, Nucharee Tuntisuwanno, Khontaros Chaiyasut, Sartjin Peerajan, Chaiyavat Chaiyasut
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Published: 2018
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http://cmuir.cmu.ac.th/jspui/handle/6653943832/58882
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Institution: Chiang Mai University
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spelling th-cmuir.6653943832-588822018-09-05T04:37:59Z Development and stability evaluation of vaginal suppository containing glycyrrhiza glabra L. For the treatment of Candida albicans infection Sasithorn Sirilun Bhagavathi Sundaram Sivamaruthi Periyanaina Kesika Noppawat Pengkumsri Nucharee Tuntisuwanno Khontaros Chaiyasut Sartjin Peerajan Chaiyavat Chaiyasut Medicine Pharmacology, Toxicology and Pharmaceutics © 2018 The Authors. Objective: The present study assessed the antioxidant activity, phytochemical content, and anti-Candida albicans property of ethanolic extract of Glycyrrhiza glabra L. (licorice). In addition, suppository formula (SF) was developed with licorice extract (LE), and the stability of SF was evaluated. Methods: The total phenolic and flavonoid content was measured by colorimetric methods. 2, 2′-azino-bis-3-ethylbenzthiazoline-6-sulfonic acid (ABTS), ferric reducing antioxidant power (FRAP), inhibition of lipid peroxidation (LPO), nitric oxide (NO), and superoxide (SO) radical scavenging assays were performed to evaluate antioxidant property. Antimicrobial activity was determined by plating method. The active principle was determined by high-performance liquid chromatography method. Results: The licorice sample was extracted with 95% ethanol, and 26.91±1.35% of yield was observed. The LE contains phenolic acids (167.70±3.18 mg gallic acid equivalent/g extract), flavonoids (162.53±9.95 mg quercetin equivalent/g extract), and glabridin (3.90±0.05 mg/g extract). LE exhibited the scavenging activity in terms of 810.53±25.37 µM of Trolox equivalent/mg of extract, 165.04±5.10 µM of FeSO4equivalent/mg of extract, and 3750.35±1.25, 68.25±0.07, and 511±0.80 µM of Trolox equivalent/mg of extract in ABTS, FRAP, LPO, NO, and SO assays, respectively. About ≥62.50, ≥125, and ≥250 µg/mL of LE was recorded as minimal inhibitory concentration against C. albicans, Lactobacillus casei, and Lactobacillus acidophilus, respectively. The SF was formulated and the stability was evaluated. The activity and color of SFs did not differ on storage. Moreover, no spot formation was observed. Conclusion: The SF with LE could be the safe therapeutic strategy for the treatment of candida infection in vaginal region. 2018-09-05T04:34:35Z 2018-09-05T04:34:35Z 2018-07-01 Journal 24553891 09742441 2-s2.0-85049870274 10.22159/ajpcr.2018.v11i7.25927 https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85049870274&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/58882
institution Chiang Mai University
building Chiang Mai University Library
country Thailand
collection CMU Intellectual Repository
topic Medicine
Pharmacology, Toxicology and Pharmaceutics
spellingShingle Medicine
Pharmacology, Toxicology and Pharmaceutics
Sasithorn Sirilun
Bhagavathi Sundaram Sivamaruthi
Periyanaina Kesika
Noppawat Pengkumsri
Nucharee Tuntisuwanno
Khontaros Chaiyasut
Sartjin Peerajan
Chaiyavat Chaiyasut
Development and stability evaluation of vaginal suppository containing glycyrrhiza glabra L. For the treatment of Candida albicans infection
description © 2018 The Authors. Objective: The present study assessed the antioxidant activity, phytochemical content, and anti-Candida albicans property of ethanolic extract of Glycyrrhiza glabra L. (licorice). In addition, suppository formula (SF) was developed with licorice extract (LE), and the stability of SF was evaluated. Methods: The total phenolic and flavonoid content was measured by colorimetric methods. 2, 2′-azino-bis-3-ethylbenzthiazoline-6-sulfonic acid (ABTS), ferric reducing antioxidant power (FRAP), inhibition of lipid peroxidation (LPO), nitric oxide (NO), and superoxide (SO) radical scavenging assays were performed to evaluate antioxidant property. Antimicrobial activity was determined by plating method. The active principle was determined by high-performance liquid chromatography method. Results: The licorice sample was extracted with 95% ethanol, and 26.91±1.35% of yield was observed. The LE contains phenolic acids (167.70±3.18 mg gallic acid equivalent/g extract), flavonoids (162.53±9.95 mg quercetin equivalent/g extract), and glabridin (3.90±0.05 mg/g extract). LE exhibited the scavenging activity in terms of 810.53±25.37 µM of Trolox equivalent/mg of extract, 165.04±5.10 µM of FeSO4equivalent/mg of extract, and 3750.35±1.25, 68.25±0.07, and 511±0.80 µM of Trolox equivalent/mg of extract in ABTS, FRAP, LPO, NO, and SO assays, respectively. About ≥62.50, ≥125, and ≥250 µg/mL of LE was recorded as minimal inhibitory concentration against C. albicans, Lactobacillus casei, and Lactobacillus acidophilus, respectively. The SF was formulated and the stability was evaluated. The activity and color of SFs did not differ on storage. Moreover, no spot formation was observed. Conclusion: The SF with LE could be the safe therapeutic strategy for the treatment of candida infection in vaginal region.
format Journal
author Sasithorn Sirilun
Bhagavathi Sundaram Sivamaruthi
Periyanaina Kesika
Noppawat Pengkumsri
Nucharee Tuntisuwanno
Khontaros Chaiyasut
Sartjin Peerajan
Chaiyavat Chaiyasut
author_facet Sasithorn Sirilun
Bhagavathi Sundaram Sivamaruthi
Periyanaina Kesika
Noppawat Pengkumsri
Nucharee Tuntisuwanno
Khontaros Chaiyasut
Sartjin Peerajan
Chaiyavat Chaiyasut
author_sort Sasithorn Sirilun
title Development and stability evaluation of vaginal suppository containing glycyrrhiza glabra L. For the treatment of Candida albicans infection
title_short Development and stability evaluation of vaginal suppository containing glycyrrhiza glabra L. For the treatment of Candida albicans infection
title_full Development and stability evaluation of vaginal suppository containing glycyrrhiza glabra L. For the treatment of Candida albicans infection
title_fullStr Development and stability evaluation of vaginal suppository containing glycyrrhiza glabra L. For the treatment of Candida albicans infection
title_full_unstemmed Development and stability evaluation of vaginal suppository containing glycyrrhiza glabra L. For the treatment of Candida albicans infection
title_sort development and stability evaluation of vaginal suppository containing glycyrrhiza glabra l. for the treatment of candida albicans infection
publishDate 2018
url https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85049870274&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/58882
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