Analysis of real-time SYBR-polymerase chain reaction cycle threshold for diagnosis of the α-thalassemia-1 Southeast Asian type deletion: Application to carrier screening and prenatal diagnosis of Hb Bart's hydrops fetalis

Without gel electrophoresis and specific probes, the two tubes real-time SYBR-polymerase chain reaction (SYBR-PCR) was setup by using different primer sets: P1/P2 for the detection of wild type α-globin gene alleles and P1/P3 for detection of the allele bearing the Southeast Asian (SEA) type (- -SEA...

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Main Authors: Sakorn Pornprasert, Kanyakan Sukunthamala, Jittraporn Sacome, Arunee Phusua, Rattika Saetung, Torpong Sanguansermsri, Pranee Leechanachai
Format: Journal
Published: 2018
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http://cmuir.cmu.ac.th/jspui/handle/6653943832/60161
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Institution: Chiang Mai University
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spelling th-cmuir.6653943832-601612018-09-10T03:46:11Z Analysis of real-time SYBR-polymerase chain reaction cycle threshold for diagnosis of the α-thalassemia-1 Southeast Asian type deletion: Application to carrier screening and prenatal diagnosis of Hb Bart's hydrops fetalis Sakorn Pornprasert Kanyakan Sukunthamala Jittraporn Sacome Arunee Phusua Rattika Saetung Torpong Sanguansermsri Pranee Leechanachai Biochemistry, Genetics and Molecular Biology Medicine Without gel electrophoresis and specific probes, the two tubes real-time SYBR-polymerase chain reaction (SYBR-PCR) was setup by using different primer sets: P1/P2 for the detection of wild type α-globin gene alleles and P1/P3 for detection of the allele bearing the Southeast Asian (SEA) type (- -SEA) deletion. Analyses of the cycle threshold (CT) values obtained by each primer set together with a delta-cycle threshold (ΔCT) and CT ratio, showed that lower CT values generated by primer sets P1/P2 and P1/P3 were observed in normal and Hb Bart's hydrops fetalis subjects, respectively. In heterozygous subjects the CT values generated by both sets of primers were similar to each other. There was no overlapping of ΔCT and CT ratio between normal, heterozygous and Hb Bart's hydrops fetalis subjects. Therefore, the two tubes real-time SYBR-PCR could represent a rapid, cost effective, high-throughput assay for screening of carriers and prenatal diagnosis of α-thalassemia-1 (α-thal-1) with the SEA type (- -SEA) deletion. Copyright © Informa Healthcare USA, Inc. 2018-09-10T03:38:51Z 2018-09-10T03:38:51Z 2008-07-01 Journal 1532432X 03630269 2-s2.0-43849101714 10.1080/03630260701798367 https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=43849101714&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/60161
institution Chiang Mai University
building Chiang Mai University Library
country Thailand
collection CMU Intellectual Repository
topic Biochemistry, Genetics and Molecular Biology
Medicine
spellingShingle Biochemistry, Genetics and Molecular Biology
Medicine
Sakorn Pornprasert
Kanyakan Sukunthamala
Jittraporn Sacome
Arunee Phusua
Rattika Saetung
Torpong Sanguansermsri
Pranee Leechanachai
Analysis of real-time SYBR-polymerase chain reaction cycle threshold for diagnosis of the α-thalassemia-1 Southeast Asian type deletion: Application to carrier screening and prenatal diagnosis of Hb Bart's hydrops fetalis
description Without gel electrophoresis and specific probes, the two tubes real-time SYBR-polymerase chain reaction (SYBR-PCR) was setup by using different primer sets: P1/P2 for the detection of wild type α-globin gene alleles and P1/P3 for detection of the allele bearing the Southeast Asian (SEA) type (- -SEA) deletion. Analyses of the cycle threshold (CT) values obtained by each primer set together with a delta-cycle threshold (ΔCT) and CT ratio, showed that lower CT values generated by primer sets P1/P2 and P1/P3 were observed in normal and Hb Bart's hydrops fetalis subjects, respectively. In heterozygous subjects the CT values generated by both sets of primers were similar to each other. There was no overlapping of ΔCT and CT ratio between normal, heterozygous and Hb Bart's hydrops fetalis subjects. Therefore, the two tubes real-time SYBR-PCR could represent a rapid, cost effective, high-throughput assay for screening of carriers and prenatal diagnosis of α-thalassemia-1 (α-thal-1) with the SEA type (- -SEA) deletion. Copyright © Informa Healthcare USA, Inc.
format Journal
author Sakorn Pornprasert
Kanyakan Sukunthamala
Jittraporn Sacome
Arunee Phusua
Rattika Saetung
Torpong Sanguansermsri
Pranee Leechanachai
author_facet Sakorn Pornprasert
Kanyakan Sukunthamala
Jittraporn Sacome
Arunee Phusua
Rattika Saetung
Torpong Sanguansermsri
Pranee Leechanachai
author_sort Sakorn Pornprasert
title Analysis of real-time SYBR-polymerase chain reaction cycle threshold for diagnosis of the α-thalassemia-1 Southeast Asian type deletion: Application to carrier screening and prenatal diagnosis of Hb Bart's hydrops fetalis
title_short Analysis of real-time SYBR-polymerase chain reaction cycle threshold for diagnosis of the α-thalassemia-1 Southeast Asian type deletion: Application to carrier screening and prenatal diagnosis of Hb Bart's hydrops fetalis
title_full Analysis of real-time SYBR-polymerase chain reaction cycle threshold for diagnosis of the α-thalassemia-1 Southeast Asian type deletion: Application to carrier screening and prenatal diagnosis of Hb Bart's hydrops fetalis
title_fullStr Analysis of real-time SYBR-polymerase chain reaction cycle threshold for diagnosis of the α-thalassemia-1 Southeast Asian type deletion: Application to carrier screening and prenatal diagnosis of Hb Bart's hydrops fetalis
title_full_unstemmed Analysis of real-time SYBR-polymerase chain reaction cycle threshold for diagnosis of the α-thalassemia-1 Southeast Asian type deletion: Application to carrier screening and prenatal diagnosis of Hb Bart's hydrops fetalis
title_sort analysis of real-time sybr-polymerase chain reaction cycle threshold for diagnosis of the α-thalassemia-1 southeast asian type deletion: application to carrier screening and prenatal diagnosis of hb bart's hydrops fetalis
publishDate 2018
url https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=43849101714&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/60161
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