Analysis of real-time SYBR-polymerase chain reaction cycle threshold for diagnosis of the α-thalassemia-1 Southeast Asian type deletion: Application to carrier screening and prenatal diagnosis of Hb Bart's hydrops fetalis

Analysis of real-time SYBR-polymerase chain reaction cycle threshold for diagnosis of the α-thalassemia-1 Southeast Asian type deletion: Application to carrier screening and prenatal diagnosis of Hb Bart's hydrops fetalis

Without gel electrophoresis and specific probes, the two tubes real-time SYBR-polymerase chain reaction (SYBR-PCR) was setup by using different primer sets: P1/P2 for the detection of wild type α-globin gene alleles and P1/P3 for detection of the allele bearing the Southeast Asian (SEA) type (- -SEA...

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Bibliographic Details
Main Authors: Sakorn Pornprasert, Kanyakan Sukunthamala, Jittraporn Sacome, Arunee Phusua, Rattika Saetung, Torpong Sanguansermsri, Pranee Leechanachai
Format: Journal
Published: 2018
Subjects:
Biochemistry, Genetics and Molecular Biology
Medicine
Online Access:https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=43849101714&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/60161
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Institution: Chiang Mai University

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