Analysis of real-time SYBR-polymerase chain reaction cycle threshold for diagnosis of the α-thalassemia-1 Southeast Asian type deletion: Application to carrier screening and prenatal diagnosis of Hb Bart's hydrops fetalis

• Main Authors: Sakorn Pornprasert, Kanyakan Sukunthamala, Jittraporn Sacome, Arunee Phusua, Rattika Saetung, Torpong Sanguansermsri, Pranee Leechanachai
• Format: Journal
• Published: 2018
• Subjects: Biochemistry, Genetics and Molecular Biology; Medicine
• Online Access: https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=43849101714&origin=inward; http://cmuir.cmu.ac.th/jspui/handle/6653943832/60161
• Institution: Chiang Mai University

Without gel electrophoresis and specific probes, the two tubes real-time SYBR-polymerase chain reaction (SYBR-PCR) was setup by using different primer sets: P1/P2 for the detection of wild type α-globin gene alleles and P1/P3 for detection of the allele bearing the Southeast Asian (SEA) type (- -SEA...