Cloning and characterization of a novel trimethoprim-resistant dihydrofolate reductase from a nosocomial isolate of Staphylococcus aureus CM.S2 (IMCJ1454)

Cloning and characterization of a novel trimethoprim-resistant dihydrofolate reductase from a nosocomial isolate of Staphylococcus aureus CM.S2 (IMCJ1454)

A novel gene, dfrG, encoding a trimethoprim (TMP)-resistant dihydrofolate reductase (DHFR, designated S3DHFR) was cloned from a clinical isolate of methicillin-resistant Staphylococcus aureus. Escherichia coli expressing dfrG was highly resistant to TMP. Recombinant S3DHFR exhibited DHFR activity th...

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Bibliographic Details
Main Authors: Jun Ichiro Sekiguchi, Prasit Tharavichitkul, Tohru Miyoshi-Akiyama, Vena Chupia, Tomoko Fujino, Minako Araake, Atsushi Irie, Koji Morita, Tadatoshi Kuratsuji, Teruo Kirikae
Format: Journal
Published: 2018
Subjects:
Medicine
Pharmacology, Toxicology and Pharmaceutics
Online Access:https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=24144502081&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/62352
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Institution: Chiang Mai University
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Summary:A novel gene, dfrG, encoding a trimethoprim (TMP)-resistant dihydrofolate reductase (DHFR, designated S3DHFR) was cloned from a clinical isolate of methicillin-resistant Staphylococcus aureus. Escherichia coli expressing dfrG was highly resistant to TMP. Recombinant S3DHFR exhibited DHFR activity that was not inhibited by TMP. Copyright © 2005, American Society for Microbiology. All Rights Reserved.

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