Cloning and characterization of a novel trimethoprim-resistant dihydrofolate reductase from a nosocomial isolate of Staphylococcus aureus CM.S2 (IMCJ1454)

A novel gene, dfrG, encoding a trimethoprim (TMP)-resistant dihydrofolate reductase (DHFR, designated S3DHFR) was cloned from a clinical isolate of methicillin-resistant Staphylococcus aureus. Escherichia coli expressing dfrG was highly resistant to TMP. Recombinant S3DHFR exhibited DHFR activity th...

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Main Authors:	Jun Ichiro Sekiguchi, Prasit Tharavichitkul, Tohru Miyoshi-Akiyama, Vena Chupia, Tomoko Fujino, Minako Araake, Atsushi Irie, Koji Morita, Tadatoshi Kuratsuji, Teruo Kirikae
Format:	Journal
Published:	2018
Subjects:	Medicine Pharmacology, Toxicology and Pharmaceutics
Online Access:	https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=24144502081&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/62352
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Institution:	Chiang Mai University

id	th-cmuir.6653943832-62352
record_format	dspace
spelling	th-cmuir.6653943832-623522018-09-11T09:27:47Z Cloning and characterization of a novel trimethoprim-resistant dihydrofolate reductase from a nosocomial isolate of Staphylococcus aureus CM.S2 (IMCJ1454) Jun Ichiro Sekiguchi Prasit Tharavichitkul Tohru Miyoshi-Akiyama Vena Chupia Tomoko Fujino Minako Araake Atsushi Irie Koji Morita Tadatoshi Kuratsuji Teruo Kirikae Medicine Pharmacology, Toxicology and Pharmaceutics A novel gene, dfrG, encoding a trimethoprim (TMP)-resistant dihydrofolate reductase (DHFR, designated S3DHFR) was cloned from a clinical isolate of methicillin-resistant Staphylococcus aureus. Escherichia coli expressing dfrG was highly resistant to TMP. Recombinant S3DHFR exhibited DHFR activity that was not inhibited by TMP. Copyright © 2005, American Society for Microbiology. All Rights Reserved. 2018-09-11T09:26:05Z 2018-09-11T09:26:05Z 2005-09-01 Journal 00664804 2-s2.0-24144502081 10.1128/AAC.49.9.3948-3951.2005 https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=24144502081&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/62352
institution	Chiang Mai University
building	Chiang Mai University Library
country	Thailand
collection	CMU Intellectual Repository
topic	Medicine Pharmacology, Toxicology and Pharmaceutics
spellingShingle	Medicine Pharmacology, Toxicology and Pharmaceutics Jun Ichiro Sekiguchi Prasit Tharavichitkul Tohru Miyoshi-Akiyama Vena Chupia Tomoko Fujino Minako Araake Atsushi Irie Koji Morita Tadatoshi Kuratsuji Teruo Kirikae Cloning and characterization of a novel trimethoprim-resistant dihydrofolate reductase from a nosocomial isolate of Staphylococcus aureus CM.S2 (IMCJ1454)
description	A novel gene, dfrG, encoding a trimethoprim (TMP)-resistant dihydrofolate reductase (DHFR, designated S3DHFR) was cloned from a clinical isolate of methicillin-resistant Staphylococcus aureus. Escherichia coli expressing dfrG was highly resistant to TMP. Recombinant S3DHFR exhibited DHFR activity that was not inhibited by TMP. Copyright © 2005, American Society for Microbiology. All Rights Reserved.
format	Journal
author	Jun Ichiro Sekiguchi Prasit Tharavichitkul Tohru Miyoshi-Akiyama Vena Chupia Tomoko Fujino Minako Araake Atsushi Irie Koji Morita Tadatoshi Kuratsuji Teruo Kirikae
author_facet	Jun Ichiro Sekiguchi Prasit Tharavichitkul Tohru Miyoshi-Akiyama Vena Chupia Tomoko Fujino Minako Araake Atsushi Irie Koji Morita Tadatoshi Kuratsuji Teruo Kirikae
author_sort	Jun Ichiro Sekiguchi
title	Cloning and characterization of a novel trimethoprim-resistant dihydrofolate reductase from a nosocomial isolate of Staphylococcus aureus CM.S2 (IMCJ1454)
title_short	Cloning and characterization of a novel trimethoprim-resistant dihydrofolate reductase from a nosocomial isolate of Staphylococcus aureus CM.S2 (IMCJ1454)
title_full	Cloning and characterization of a novel trimethoprim-resistant dihydrofolate reductase from a nosocomial isolate of Staphylococcus aureus CM.S2 (IMCJ1454)
title_fullStr	Cloning and characterization of a novel trimethoprim-resistant dihydrofolate reductase from a nosocomial isolate of Staphylococcus aureus CM.S2 (IMCJ1454)
title_full_unstemmed	Cloning and characterization of a novel trimethoprim-resistant dihydrofolate reductase from a nosocomial isolate of Staphylococcus aureus CM.S2 (IMCJ1454)
title_sort	cloning and characterization of a novel trimethoprim-resistant dihydrofolate reductase from a nosocomial isolate of staphylococcus aureus cm.s2 (imcj1454)
publishDate	2018
url	https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=24144502081&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/62352
_version_	1681425790862884864

Cloning and characterization of a novel trimethoprim-resistant dihydrofolate reductase from a nosocomial isolate of Staphylococcus aureus CM.S2 (IMCJ1454)

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