Cloning and characterization of a novel trimethoprim-resistant dihydrofolate reductase from a nosocomial isolate of Staphylococcus aureus CM.S2 (IMCJ1454)

• Main Authors: Jun Ichiro Sekiguchi, Prasit Tharavichitkul, Tohru Miyoshi-Akiyama, Vena Chupia, Tomoko Fujino, Minako Araake, Atsushi Irie, Koji Morita, Tadatoshi Kuratsuji, Teruo Kirikae
• Format: Journal
• Published: 2018
• Subjects: Medicine; Pharmacology, Toxicology and Pharmaceutics
• Online Access: https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=24144502081&origin=inward; http://cmuir.cmu.ac.th/jspui/handle/6653943832/62352
• Institution: Chiang Mai University

A novel gene, dfrG, encoding a trimethoprim (TMP)-resistant dihydrofolate reductase (DHFR, designated S3DHFR) was cloned from a clinical isolate of methicillin-resistant Staphylococcus aureus. Escherichia coli expressing dfrG was highly resistant to TMP. Recombinant S3DHFR exhibited DHFR activity th...