Molecular structures, chemical properties and biological activities of polysaccharide from Smilax glabra rhizome
© 2018 The water-soluble crude polysaccharides, extracted from the rhizome of Smilax glabra, were fractionated using an anion exchange chromatography, yielding two fractions, F1 and F2. The crude and fractions (F1 and F2) mainly consisted of carbohydrates (66.7%–91.1%), proteins (7.30%–23.9%) and mi...
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th-cmuir.6653943832-625522018-11-29T07:32:13Z Molecular structures, chemical properties and biological activities of polysaccharide from Smilax glabra rhizome Je Young Lee Changsheng Li Utoomporn Surayot Khamphone Yelithao Sang Min Lee Woo Jung Park Mehdi Tabarsa Sang Guan You Biochemistry, Genetics and Molecular Biology © 2018 The water-soluble crude polysaccharides, extracted from the rhizome of Smilax glabra, were fractionated using an anion exchange chromatography, yielding two fractions, F1 and F2. The crude and fractions (F1 and F2) mainly consisted of carbohydrates (66.7%–91.1%), proteins (7.30%–23.9%) and minor amount of sulfates (1.60%–9.40%). Glucose was the major monosaccharide unit of the polysaccharides with different levels of sugar constituents including galactose, arabinose, rhamnose and mannose. The molecular weight (Mw) of crude and fractions ranged from 32,102–6.3 × 103 g/mol. The crude and fractions could stimulate RAW264.7 cells to release nitric oxide and cytokines via up-regulation of their mRNA expression by the activation of NF-κB and MAPKs pathways. The related pattern recognition receptors (PRRs) on the surface of the cells appeared to be TLR2 and CR3. The GC–MS analysis revealed that the main backbone of the most immune-enhancing F2 was (1 → 4)-linked glucose and galactose chain with minor linkages of (1 → 6)-galactose, (1 → 3)-mannose, (1 → 2)-rhamnose and (1 → 5)-arabinose with some branches at C-3 and C-4 rhamnose, or C-6 galactose. 2018-11-29T07:32:13Z 2018-11-29T07:32:13Z 2018-12-01 Journal 18790003 01418130 2-s2.0-85054295520 10.1016/j.ijbiomac.2018.09.138 https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85054295520&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/62552 |
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Biochemistry, Genetics and Molecular Biology Je Young Lee Changsheng Li Utoomporn Surayot Khamphone Yelithao Sang Min Lee Woo Jung Park Mehdi Tabarsa Sang Guan You Molecular structures, chemical properties and biological activities of polysaccharide from Smilax glabra rhizome |
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© 2018 The water-soluble crude polysaccharides, extracted from the rhizome of Smilax glabra, were fractionated using an anion exchange chromatography, yielding two fractions, F1 and F2. The crude and fractions (F1 and F2) mainly consisted of carbohydrates (66.7%–91.1%), proteins (7.30%–23.9%) and minor amount of sulfates (1.60%–9.40%). Glucose was the major monosaccharide unit of the polysaccharides with different levels of sugar constituents including galactose, arabinose, rhamnose and mannose. The molecular weight (Mw) of crude and fractions ranged from 32,102–6.3 × 103 g/mol. The crude and fractions could stimulate RAW264.7 cells to release nitric oxide and cytokines via up-regulation of their mRNA expression by the activation of NF-κB and MAPKs pathways. The related pattern recognition receptors (PRRs) on the surface of the cells appeared to be TLR2 and CR3. The GC–MS analysis revealed that the main backbone of the most immune-enhancing F2 was (1 → 4)-linked glucose and galactose chain with minor linkages of (1 → 6)-galactose, (1 → 3)-mannose, (1 → 2)-rhamnose and (1 → 5)-arabinose with some branches at C-3 and C-4 rhamnose, or C-6 galactose. |
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author |
Je Young Lee Changsheng Li Utoomporn Surayot Khamphone Yelithao Sang Min Lee Woo Jung Park Mehdi Tabarsa Sang Guan You |
author_facet |
Je Young Lee Changsheng Li Utoomporn Surayot Khamphone Yelithao Sang Min Lee Woo Jung Park Mehdi Tabarsa Sang Guan You |
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Je Young Lee |
title |
Molecular structures, chemical properties and biological activities of polysaccharide from Smilax glabra rhizome |
title_short |
Molecular structures, chemical properties and biological activities of polysaccharide from Smilax glabra rhizome |
title_full |
Molecular structures, chemical properties and biological activities of polysaccharide from Smilax glabra rhizome |
title_fullStr |
Molecular structures, chemical properties and biological activities of polysaccharide from Smilax glabra rhizome |
title_full_unstemmed |
Molecular structures, chemical properties and biological activities of polysaccharide from Smilax glabra rhizome |
title_sort |
molecular structures, chemical properties and biological activities of polysaccharide from smilax glabra rhizome |
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2018 |
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https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85054295520&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/62552 |
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