Influences of Buffer Systems on Enzymatic Saccharification of Rice Husk Holocellulose and Fermenting Ability of Various Ethanol Producing Microorganisms

Influences of Buffer Systems on Enzymatic Saccharification of Rice Husk Holocellulose and Fermenting Ability of Various Ethanol Producing Microorganisms

Four saccharification buffers, including acetate, citrate, phthalate and succinate buffers, were evaluated for their effects on both saccharification and fermentation of rice husk holocellulose. At a concentration of 30 mM, all buffer systems were found to be suitable for saccharification by three c...

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Main Authors: Woottichai Nachaiwieng, Apinun Kanpiengjai, Takashi Watanabe, Chartchai Khanongnuch
Format: บทความวารสาร
Language:English
Published: Science Faculty of Chiang Mai University 2019
Online Access:http://it.science.cmu.ac.th/ejournal/dl.php?journal_id=8016
http://cmuir.cmu.ac.th/jspui/handle/6653943832/63867
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Institution: Chiang Mai University
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spelling th-cmuir.6653943832-638672019-05-07T09:59:36Z Influences of Buffer Systems on Enzymatic Saccharification of Rice Husk Holocellulose and Fermenting Ability of Various Ethanol Producing Microorganisms Woottichai Nachaiwieng Apinun Kanpiengjai Takashi Watanabe Chartchai Khanongnuch Four saccharification buffers, including acetate, citrate, phthalate and succinate buffers, were evaluated for their effects on both saccharification and fermentation of rice husk holocellulose. At a concentration of 30 mM, all buffer systems were found to be suitable for saccharification by three commercial cellulolytic enzymes (Meicelase, Accellerase 1500 and Accellerase XC). However, at concentrations of 50-200 mM, citrate and acetate buffers partially inhibited the fermenting ability of Saccharomyces cerevisiase, Zymomonas mobilis, Kluyveromyces marxianus, Candida shehatae and Pichia stipitis. This was in contrast to phthalate and succinate buffers in which these organisms retained 100% relative fermenting ability within concentrations in the range of 50-500 mM. This is the first report indicating potential buffers appropriate for broad ethanol producing microorganisms in bioethanol fermentation, particularly for co-culture fermentation of cellulosic hydrolysate. 2019-05-07T09:59:36Z 2019-05-07T09:59:36Z 2017 บทความวารสาร 0125-2526 http://it.science.cmu.ac.th/ejournal/dl.php?journal_id=8016 http://cmuir.cmu.ac.th/jspui/handle/6653943832/63867 Eng Science Faculty of Chiang Mai University
institution Chiang Mai University
building Chiang Mai University Library
country Thailand
collection CMU Intellectual Repository
language English
description Four saccharification buffers, including acetate, citrate, phthalate and succinate buffers, were evaluated for their effects on both saccharification and fermentation of rice husk holocellulose. At a concentration of 30 mM, all buffer systems were found to be suitable for saccharification by three commercial cellulolytic enzymes (Meicelase, Accellerase 1500 and Accellerase XC). However, at concentrations of 50-200 mM, citrate and acetate buffers partially inhibited the fermenting ability of Saccharomyces cerevisiase, Zymomonas mobilis, Kluyveromyces marxianus, Candida shehatae and Pichia stipitis. This was in contrast to phthalate and succinate buffers in which these organisms retained 100% relative fermenting ability within concentrations in the range of 50-500 mM. This is the first report indicating potential buffers appropriate for broad ethanol producing microorganisms in bioethanol fermentation, particularly for co-culture fermentation of cellulosic hydrolysate.
format บทความวารสาร
author Woottichai Nachaiwieng
Apinun Kanpiengjai
Takashi Watanabe
Chartchai Khanongnuch
spellingShingle Woottichai Nachaiwieng
Apinun Kanpiengjai
Takashi Watanabe
Chartchai Khanongnuch
Influences of Buffer Systems on Enzymatic Saccharification of Rice Husk Holocellulose and Fermenting Ability of Various Ethanol Producing Microorganisms
author_facet Woottichai Nachaiwieng
Apinun Kanpiengjai
Takashi Watanabe
Chartchai Khanongnuch
author_sort Woottichai Nachaiwieng
title Influences of Buffer Systems on Enzymatic Saccharification of Rice Husk Holocellulose and Fermenting Ability of Various Ethanol Producing Microorganisms
title_short Influences of Buffer Systems on Enzymatic Saccharification of Rice Husk Holocellulose and Fermenting Ability of Various Ethanol Producing Microorganisms
title_full Influences of Buffer Systems on Enzymatic Saccharification of Rice Husk Holocellulose and Fermenting Ability of Various Ethanol Producing Microorganisms
title_fullStr Influences of Buffer Systems on Enzymatic Saccharification of Rice Husk Holocellulose and Fermenting Ability of Various Ethanol Producing Microorganisms
title_full_unstemmed Influences of Buffer Systems on Enzymatic Saccharification of Rice Husk Holocellulose and Fermenting Ability of Various Ethanol Producing Microorganisms
title_sort influences of buffer systems on enzymatic saccharification of rice husk holocellulose and fermenting ability of various ethanol producing microorganisms
publisher Science Faculty of Chiang Mai University
publishDate 2019
url http://it.science.cmu.ac.th/ejournal/dl.php?journal_id=8016
http://cmuir.cmu.ac.th/jspui/handle/6653943832/63867
_version_ 1681425975174234112

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