Preparation of Isoflavone glucosides from Soy germ and β-Glucosidase from Bacillus coagulans PR03 for Isoflavone aglycones Production

© 2019, Chiang Mai University. This research optimized isoflavone aglycones production from soy germ. The processes included isoflavone glucosides extraction from soy germ to use as a precursor for isoflavone aglycones production. β-glucosidase was produced from B. coagulans PR03 and used to convert...

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Main Authors: Supakit Chaipoot, Rewat Phongphisutthinant, Sujinda Sriwattana, Sakaewan Ounjaijean, Pairote Wiriyacharee
Format: Journal
Published: 2020
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http://cmuir.cmu.ac.th/jspui/handle/6653943832/68134
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spelling th-cmuir.6653943832-681342020-04-02T15:21:27Z Preparation of Isoflavone glucosides from Soy germ and β-Glucosidase from Bacillus coagulans PR03 for Isoflavone aglycones Production Supakit Chaipoot Rewat Phongphisutthinant Sujinda Sriwattana Sakaewan Ounjaijean Pairote Wiriyacharee Multidisciplinary © 2019, Chiang Mai University. This research optimized isoflavone aglycones production from soy germ. The processes included isoflavone glucosides extraction from soy germ to use as a precursor for isoflavone aglycones production. β-glucosidase was produced from B. coagulans PR03 and used to convert glucosides form to aglycones. For the β-glucosidase production using Plackett and Burman Design (n=8) and 22 factorial experiment with central composite design, the suitable medium containing peptone (2.00%), beef extract (14.84%), glucose (2.00%) and magnesium sulphate (0.10%) with pH 7.96 was used and incubated at 30 °C. The resulting β-glucosidase activity was 4.01 mU/ml. However, the isoflavone glucosides was extracted by use of soy germ in 80% ethanol at a ratio of 1:5 with high power ultrasonication technique at 80 oC for 160 min using a completely randomized design. The extracted isoflavone glucosides contained daidzin, genistin and glycitin of 307.47, 214.84 and 73.63 mg/100 g. (dry basis), respectively. Finally, isoflavone aglycones production was optimized using 22 factorial experiment with central composite design, the total isoflavone glucosides (595.95 mg/100 g. dry basis) was convert to isoflavone aglycones (112.90 μg/ml) by β-glucosidase at 37.5 °C for 120 hours. 2020-04-02T15:21:27Z 2020-04-02T15:21:27Z 2019-01-01 Journal 16851994 2-s2.0-85073944239 10.12982/CMUJNS.2019.0032 https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85073944239&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/68134
institution Chiang Mai University
building Chiang Mai University Library
country Thailand
collection CMU Intellectual Repository
topic Multidisciplinary
spellingShingle Multidisciplinary
Supakit Chaipoot
Rewat Phongphisutthinant
Sujinda Sriwattana
Sakaewan Ounjaijean
Pairote Wiriyacharee
Preparation of Isoflavone glucosides from Soy germ and β-Glucosidase from Bacillus coagulans PR03 for Isoflavone aglycones Production
description © 2019, Chiang Mai University. This research optimized isoflavone aglycones production from soy germ. The processes included isoflavone glucosides extraction from soy germ to use as a precursor for isoflavone aglycones production. β-glucosidase was produced from B. coagulans PR03 and used to convert glucosides form to aglycones. For the β-glucosidase production using Plackett and Burman Design (n=8) and 22 factorial experiment with central composite design, the suitable medium containing peptone (2.00%), beef extract (14.84%), glucose (2.00%) and magnesium sulphate (0.10%) with pH 7.96 was used and incubated at 30 °C. The resulting β-glucosidase activity was 4.01 mU/ml. However, the isoflavone glucosides was extracted by use of soy germ in 80% ethanol at a ratio of 1:5 with high power ultrasonication technique at 80 oC for 160 min using a completely randomized design. The extracted isoflavone glucosides contained daidzin, genistin and glycitin of 307.47, 214.84 and 73.63 mg/100 g. (dry basis), respectively. Finally, isoflavone aglycones production was optimized using 22 factorial experiment with central composite design, the total isoflavone glucosides (595.95 mg/100 g. dry basis) was convert to isoflavone aglycones (112.90 μg/ml) by β-glucosidase at 37.5 °C for 120 hours.
format Journal
author Supakit Chaipoot
Rewat Phongphisutthinant
Sujinda Sriwattana
Sakaewan Ounjaijean
Pairote Wiriyacharee
author_facet Supakit Chaipoot
Rewat Phongphisutthinant
Sujinda Sriwattana
Sakaewan Ounjaijean
Pairote Wiriyacharee
author_sort Supakit Chaipoot
title Preparation of Isoflavone glucosides from Soy germ and β-Glucosidase from Bacillus coagulans PR03 for Isoflavone aglycones Production
title_short Preparation of Isoflavone glucosides from Soy germ and β-Glucosidase from Bacillus coagulans PR03 for Isoflavone aglycones Production
title_full Preparation of Isoflavone glucosides from Soy germ and β-Glucosidase from Bacillus coagulans PR03 for Isoflavone aglycones Production
title_fullStr Preparation of Isoflavone glucosides from Soy germ and β-Glucosidase from Bacillus coagulans PR03 for Isoflavone aglycones Production
title_full_unstemmed Preparation of Isoflavone glucosides from Soy germ and β-Glucosidase from Bacillus coagulans PR03 for Isoflavone aglycones Production
title_sort preparation of isoflavone glucosides from soy germ and β-glucosidase from bacillus coagulans pr03 for isoflavone aglycones production
publishDate 2020
url https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85073944239&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/68134
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