Production of a biologically active growth hormone from giant catfish (Pangasianodon gigas) in Escherichia coli

Production of a biologically active growth hormone from giant catfish (Pangasianodon gigas) in Escherichia coli

Giant catfish growth hormone (gcGH) cDNA was cloned and expressed in E. coli. The expected 20.5 kDa protein corresponded to the mature gcGH and was efficiently expressed. This protein was produced as inclusion bodies and comprised about 20% of total cellular proteins. The recombinant hormone promote...

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Main Authors: Boonhiang Promdonkoy, Saradee Warit, Sakol Panyim
Other Authors: Thailand National Center for Genetic Engineering and Biotechnology
Format: Article
Published: 2018
Subjects:
Biochemistry, Genetics and Molecular Biology
Online Access:https://repository.li.mahidol.ac.th/handle/123456789/21205
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Institution: Mahidol University
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spelling th-mahidol.212052018-07-24T10:37:56Z Production of a biologically active growth hormone from giant catfish (Pangasianodon gigas) in Escherichia coli Boonhiang Promdonkoy Saradee Warit Sakol Panyim Thailand National Center for Genetic Engineering and Biotechnology Mahidol University Biochemistry, Genetics and Molecular Biology Giant catfish growth hormone (gcGH) cDNA was cloned and expressed in E. coli. The expected 20.5 kDa protein corresponded to the mature gcGH and was efficiently expressed. This protein was produced as inclusion bodies and comprised about 20% of total cellular proteins. The recombinant hormone promoted growth when injected intramuscularly or intraperitoneally into goldfish (Carassius auratus) at 0.1 or 1 μg soluble gcGH per g fish body wt per week. In addition, the recombinant gcGH inclusions had growth-promoting activity similar to that of the soluble form when the fish was received either by intraperitoneal injection or by oral administration. 2018-07-24T03:37:56Z 2018-07-24T03:37:56Z 2004-04-01 Article Biotechnology Letters. Vol.26, No.8 (2004), 649-653 10.1023/B:BILE.0000023024.27549.6d 01415492 2-s2.0-3342984173 https://repository.li.mahidol.ac.th/handle/123456789/21205 Mahidol University SCOPUS https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=3342984173&origin=inward
institution Mahidol University
building Mahidol University Library
continent Asia
country Thailand
Thailand
content_provider Mahidol University Library
collection Mahidol University Institutional Repository
topic Biochemistry, Genetics and Molecular Biology
spellingShingle Biochemistry, Genetics and Molecular Biology
Boonhiang Promdonkoy
Saradee Warit
Sakol Panyim
Production of a biologically active growth hormone from giant catfish (Pangasianodon gigas) in Escherichia coli
description Giant catfish growth hormone (gcGH) cDNA was cloned and expressed in E. coli. The expected 20.5 kDa protein corresponded to the mature gcGH and was efficiently expressed. This protein was produced as inclusion bodies and comprised about 20% of total cellular proteins. The recombinant hormone promoted growth when injected intramuscularly or intraperitoneally into goldfish (Carassius auratus) at 0.1 or 1 μg soluble gcGH per g fish body wt per week. In addition, the recombinant gcGH inclusions had growth-promoting activity similar to that of the soluble form when the fish was received either by intraperitoneal injection or by oral administration.
author2 Thailand National Center for Genetic Engineering and Biotechnology
author_facet Thailand National Center for Genetic Engineering and Biotechnology
Boonhiang Promdonkoy
Saradee Warit
Sakol Panyim
format Article
author Boonhiang Promdonkoy
Saradee Warit
Sakol Panyim
author_sort Boonhiang Promdonkoy
title Production of a biologically active growth hormone from giant catfish (Pangasianodon gigas) in Escherichia coli
title_short Production of a biologically active growth hormone from giant catfish (Pangasianodon gigas) in Escherichia coli
title_full Production of a biologically active growth hormone from giant catfish (Pangasianodon gigas) in Escherichia coli
title_fullStr Production of a biologically active growth hormone from giant catfish (Pangasianodon gigas) in Escherichia coli
title_full_unstemmed Production of a biologically active growth hormone from giant catfish (Pangasianodon gigas) in Escherichia coli
title_sort production of a biologically active growth hormone from giant catfish (pangasianodon gigas) in escherichia coli
publishDate 2018
url https://repository.li.mahidol.ac.th/handle/123456789/21205
_version_ 1763491602051891200

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