Production of a biologically active growth hormone from giant catfish (Pangasianodon gigas) in Escherichia coli

• Main Authors: Boonhiang Promdonkoy, Saradee Warit, Sakol Panyim
• Other Authors: Thailand National Center for Genetic Engineering and Biotechnology
• Format: Article
• Published: 2018
• Subjects: Biochemistry, Genetics and Molecular Biology
• Online Access: https://repository.li.mahidol.ac.th/handle/123456789/21205
• Institution: Mahidol University

Giant catfish growth hormone (gcGH) cDNA was cloned and expressed in E. coli. The expected 20.5 kDa protein corresponded to the mature gcGH and was efficiently expressed. This protein was produced as inclusion bodies and comprised about 20% of total cellular proteins. The recombinant hormone promote...