Optimization of cyclodextrin production from sago starch
Cyclodextrin (CD) is synthesized by bacterial cyclodextrin glycosyltransferase (CGTase) and is widely used in food, pharmaceutical, cosmetic, and agricultural industries. In this study, Bacillus circulans CGTase was partially purified by ammonium sulfate precipitation at 50-70% saturation. The optim...
Saved in:
Main Authors: | , , , |
---|---|
Other Authors: | |
Format: | Article |
Published: |
2018
|
Subjects: | |
Online Access: | https://repository.li.mahidol.ac.th/handle/123456789/21260 |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
Institution: | Mahidol University |
id |
th-mahidol.21260 |
---|---|
record_format |
dspace |
spelling |
th-mahidol.212602018-07-24T10:41:51Z Optimization of cyclodextrin production from sago starch Nisanart Charoenlap Saovanee Dharmsthiti Sarote Sirisansaneeyakul Sittiwat Lertsiri Mahidol University Kasetsart University Chemical Engineering Energy Environmental Science Cyclodextrin (CD) is synthesized by bacterial cyclodextrin glycosyltransferase (CGTase) and is widely used in food, pharmaceutical, cosmetic, and agricultural industries. In this study, Bacillus circulans CGTase was partially purified by ammonium sulfate precipitation at 50-70% saturation. The optimum pH and temperature for CD production from sago starch were found to be in the ranges of 4.5-5.0 and 55-60°C, respectively. β-CD was the predominant product, constituting 65% of all CD products. The β-CD produced using partially purified and crude CGTase were compared and found to have no significant difference in yield and productivity. The appropriate proportion of CGTase to sago starch for β-CD production was determined by response surface methodology. The most appropriate enzyme:substrate ratio was 50 Ug sago starch-1CGTase and 60 gl-1sago starch. © 2003 Elsevier Ltd. All rights reserved. 2018-07-24T03:39:31Z 2018-07-24T03:39:31Z 2004-01-01 Article Bioresource Technology. Vol.92, No.1 (2004), 49-54 10.1016/j.biortech.2003.07.007 09608524 2-s2.0-0344393491 https://repository.li.mahidol.ac.th/handle/123456789/21260 Mahidol University SCOPUS https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=0344393491&origin=inward |
institution |
Mahidol University |
building |
Mahidol University Library |
continent |
Asia |
country |
Thailand Thailand |
content_provider |
Mahidol University Library |
collection |
Mahidol University Institutional Repository |
topic |
Chemical Engineering Energy Environmental Science |
spellingShingle |
Chemical Engineering Energy Environmental Science Nisanart Charoenlap Saovanee Dharmsthiti Sarote Sirisansaneeyakul Sittiwat Lertsiri Optimization of cyclodextrin production from sago starch |
description |
Cyclodextrin (CD) is synthesized by bacterial cyclodextrin glycosyltransferase (CGTase) and is widely used in food, pharmaceutical, cosmetic, and agricultural industries. In this study, Bacillus circulans CGTase was partially purified by ammonium sulfate precipitation at 50-70% saturation. The optimum pH and temperature for CD production from sago starch were found to be in the ranges of 4.5-5.0 and 55-60°C, respectively. β-CD was the predominant product, constituting 65% of all CD products. The β-CD produced using partially purified and crude CGTase were compared and found to have no significant difference in yield and productivity. The appropriate proportion of CGTase to sago starch for β-CD production was determined by response surface methodology. The most appropriate enzyme:substrate ratio was 50 Ug sago starch-1CGTase and 60 gl-1sago starch. © 2003 Elsevier Ltd. All rights reserved. |
author2 |
Mahidol University |
author_facet |
Mahidol University Nisanart Charoenlap Saovanee Dharmsthiti Sarote Sirisansaneeyakul Sittiwat Lertsiri |
format |
Article |
author |
Nisanart Charoenlap Saovanee Dharmsthiti Sarote Sirisansaneeyakul Sittiwat Lertsiri |
author_sort |
Nisanart Charoenlap |
title |
Optimization of cyclodextrin production from sago starch |
title_short |
Optimization of cyclodextrin production from sago starch |
title_full |
Optimization of cyclodextrin production from sago starch |
title_fullStr |
Optimization of cyclodextrin production from sago starch |
title_full_unstemmed |
Optimization of cyclodextrin production from sago starch |
title_sort |
optimization of cyclodextrin production from sago starch |
publishDate |
2018 |
url |
https://repository.li.mahidol.ac.th/handle/123456789/21260 |
_version_ |
1763492334730739712 |