Homology building as a means to define antigenic epitopes on dihydrofolate reductase (DHFR) from Plasmodium falciparum

• Main Authors: Michael Alifrangis, Inge T. Christensen, Flemming S. Jørgensen, Anita M. Rønn, Jimmy E. Weng, Ming Chen, Ib C. Bygbjerg, Worachart Sirawaraporn, Yaseelan Palarasah, Claus Koch
• Other Authors: Kobenhavns Universitet
• Format: Article
• Published: 2018
• Subjects: Immunology and Microbiology; Medicine
• Online Access: https://repository.li.mahidol.ac.th/handle/123456789/21374
• Institution: Mahidol University

Background: The aim of this study was to develop site-specific antibodies as a tool to capture Plasmodium falciparum-dihydrofolate reductase (Pf-DHFR) from blood samples from P. falciparum infected individuals in order to detect, in a sandwich ELISA, structural alterations due to point mutations in...