Optimizing bulk segregant analysis of drug resistance using Plasmodium falciparum genetic crosses conducted in humanized mice

• Main Authors: Katelyn Vendrely Brenneman, Xue Li, Sudhir Kumar, Elizabeth Delgado, Lisa A. Checkley, Douglas A. Shoue, Ann Reyes, Biley A. Abatiyow, Meseret T. Haile, Rupam Tripura, Tom Peto, Dysoley Lek, Katrina A. Button-Simons, Stefan H.I. Kappe, Mehul Dhorda, François Nosten, Standwell C. Nkhoma, Ian H. Cheeseman, Ashley M. Vaughan, Michael T. Ferdig, Tim J.C. Anderson
• Other Authors: Faculty of Tropical Medicine, Mahidol University
• Format: Article
• Published: 2022
• Subjects: Multidisciplinary
• Online Access: https://repository.li.mahidol.ac.th/handle/123456789/75457
• Institution: Mahidol University

Classical malaria parasite genetic crosses involve isolation, genotyping, and phenotyping of progeny parasites, which is time consuming and laborious. We tested a rapid alternative approach—bulk segregant analysis (BSA)—that utilizes sequencing of bulk progeny populations with and without drug selec...