Detection of labeled abasic sites in damaged DNA by capillary electrophoresis with laser-induced fluorescence

• Main Authors: Fundador, Erwin Oliver V., Rusling, James
• Format: text
• Published: Animo Repository 2007
• Subjects: Capillary electrophoresis; DNA damage; Laser-induced fluorescence; Biochemistry; Chemistry
• Online Access: https://animorepository.dlsu.edu.ph/faculty_research/5577
• Institution: De La Salle University

Removal of nucleobases from the DNA backbone leads to the formation of abasic sites. The rate of abasic site formation is significantly increased for chemically damaged nucleobases. Thus, abasic sites serve as general biomarkers for the quantification of DNA damage. Herein, we show that capillary el...