Robust RNAi enhancement via human Argonaute-2 overexpression from plasmids, viral vectors and cell lines

• Main Authors: Börner, Kathleen, Niopek, Dominik, Cotugno, Gabriella, Kaldenbach, Michaela, Pankert, Teresa, Willemsen, Joschka, Zhang, Xian, Schürmann, Nina, Streetz, Konrad, Kräusslich, Hans-Georg, Mockenhaupt, Stefan, Serva, Andrius, Grimm, Dirk, Hiet, Marie-Sophie, Wiedtke, Ellen, Binder, Marco, Castoldi, Mirco, Starkuviene, Vytaute, Erfle, Holger, Bartenschlager, Ralf, Boutros, Michael, Gilbert, Daniel F.
• Other Authors: School of Biological Sciences
• Format: Article
• Language: English
• Published: 2014
• Subjects: DRNTU::Science::Biological sciences
• Online Access: https://hdl.handle.net/10356/101612; http://hdl.handle.net/10220/18735
• Institution: Nanyang Technological University

As the only mammalian Argonaute protein capable of directly cleaving mRNAs in a small RNA-guided manner, Argonaute-2 (Ago2) is a keyplayer in RNA interference (RNAi) silencing via small interfering (si) or short hairpin (sh) RNAs. It is also a rate-limiting factor whose saturation by si/shRNAs limit...