Conformational flexibility of the dengue virus RNA-dependent RNA polymerase revealed by a complex with an inhibitor
We report a highly reproducible method to crystallize the RNA-dependent RNA polymerase (RdRp) domain of dengue virus serotype 3 (DENV-3), allowing structure refinement to a 1.79-Å resolution and revealing amino acids not seen previously. We also present a DENV-3 polymerase/inhibitor cocrystal struct...
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2015
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sg-ntu-dr.10356-1037002023-02-28T16:58:24Z Conformational flexibility of the dengue virus RNA-dependent RNA polymerase revealed by a complex with an inhibitor Yap, Lijian Lescar, Julien Noble, Christian G. Lim, Siew Pheng Chen, Yen-Liang Liew, Chong Wai Shi, Pei-Yong School of Biological Sciences DRNTU::Science::Biological sciences::Microbiology::Virology We report a highly reproducible method to crystallize the RNA-dependent RNA polymerase (RdRp) domain of dengue virus serotype 3 (DENV-3), allowing structure refinement to a 1.79-Å resolution and revealing amino acids not seen previously. We also present a DENV-3 polymerase/inhibitor cocrystal structure at a 2.1-Å resolution. The inhibitor binds to the RdRp as a dimer and causes conformational changes in the protein. The improved crystallization conditions and new structural information should accelerate structure-based drug discovery. Published version 2015-06-05T08:32:38Z 2019-12-06T21:18:19Z 2015-06-05T08:32:38Z 2019-12-06T21:18:19Z 2013 2013 Journal Article Noble, C. G., Lim, S. P., Chen, Y.-L., Liew, C. W., Yap, L., Lescar, J., et al. (2013). Conformational flexibility of the dengue virus RNA-dependent RNA polymerase revealed by a complex with an inhibitor. Journal of virology, 87(9), 5291-5295. 0022-538X https://hdl.handle.net/10356/103700 http://hdl.handle.net/10220/25795 10.1128/JVI.00045-13 23408636 en Journal of virology © 2013 American Society for Microbiology (ASM). This paper was published in Journal of Virology and is made available as an electronic reprint (preprint) with permission of American Society for Microbiology (ASM). The paper can be found at the following official DOI: [http://dx.doi.org/10.1128/JVI.00045-13]. One print or electronic copy may be made for personal use only. Systematic or multiple reproduction, distribution to multiple locations via electronic or other means, duplication of any material in this paper for a fee or for commercial purposes, or modification of the content of the paper is prohibited and is subject to penalties under law. 5 p. application/pdf |
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DRNTU::Science::Biological sciences::Microbiology::Virology Yap, Lijian Lescar, Julien Noble, Christian G. Lim, Siew Pheng Chen, Yen-Liang Liew, Chong Wai Shi, Pei-Yong Conformational flexibility of the dengue virus RNA-dependent RNA polymerase revealed by a complex with an inhibitor |
| description |
We report a highly reproducible method to crystallize the RNA-dependent RNA polymerase (RdRp) domain of dengue virus serotype 3 (DENV-3), allowing structure refinement to a 1.79-Å resolution and revealing amino acids not seen previously. We also present a DENV-3 polymerase/inhibitor cocrystal structure at a 2.1-Å resolution. The inhibitor binds to the RdRp as a dimer and causes conformational changes in the protein. The improved crystallization conditions and new structural information should accelerate structure-based drug discovery. |
| author2 |
School of Biological Sciences |
| author_facet |
School of Biological Sciences Yap, Lijian Lescar, Julien Noble, Christian G. Lim, Siew Pheng Chen, Yen-Liang Liew, Chong Wai Shi, Pei-Yong |
| format |
Article |
| author |
Yap, Lijian Lescar, Julien Noble, Christian G. Lim, Siew Pheng Chen, Yen-Liang Liew, Chong Wai Shi, Pei-Yong |
| author_sort |
Yap, Lijian |
| title |
Conformational flexibility of the dengue virus RNA-dependent RNA polymerase revealed by a complex with an inhibitor |
| title_short |
Conformational flexibility of the dengue virus RNA-dependent RNA polymerase revealed by a complex with an inhibitor |
| title_full |
Conformational flexibility of the dengue virus RNA-dependent RNA polymerase revealed by a complex with an inhibitor |
| title_fullStr |
Conformational flexibility of the dengue virus RNA-dependent RNA polymerase revealed by a complex with an inhibitor |
| title_full_unstemmed |
Conformational flexibility of the dengue virus RNA-dependent RNA polymerase revealed by a complex with an inhibitor |
| title_sort |
conformational flexibility of the dengue virus rna-dependent rna polymerase revealed by a complex with an inhibitor |
| publishDate |
2015 |
| url |
https://hdl.handle.net/10356/103700 http://hdl.handle.net/10220/25795 |
| _version_ |
1759854936283152384 |