Conformational flexibility of the dengue virus RNA-dependent RNA polymerase revealed by a complex with an inhibitor

• Main Authors: Yap, Lijian, Lescar, Julien, Noble, Christian G., Lim, Siew Pheng, Chen, Yen-Liang, Liew, Chong Wai, Shi, Pei-Yong
• Other Authors: School of Biological Sciences
• Format: Article
• Language: English
• Published: 2015
• Subjects: DRNTU::Science::Biological sciences::Microbiology::Virology
• Online Access: https://hdl.handle.net/10356/103700; http://hdl.handle.net/10220/25795
• Institution: Nanyang Technological University

We report a highly reproducible method to crystallize the RNA-dependent RNA polymerase (RdRp) domain of dengue virus serotype 3 (DENV-3), allowing structure refinement to a 1.79-Å resolution and revealing amino acids not seen previously. We also present a DENV-3 polymerase/inhibitor cocrystal struct...