CRISPR/Cpf1 facilitated large fragment deletion in Saccharomyces cerevisiae

• Main Authors: Li, Zhen-Hai, Liu, Min, Lyu, Xiao-Mei, Wang, Feng-Qing, Wei, Dong-Zhi
• Other Authors: School of Chemical and Biomedical Engineering
• Format: Article
• Language: English
• Published: 2020
• Subjects: Engineering::Bioengineering; CRISPR/Cpf1; Large Fragment Deletion
• Online Access: https://hdl.handle.net/10356/139529
• Institution: Nanyang Technological University

In this study, we focused on the applicability of CRISPR/Cpf1 in genome simplification of Saccharomyces cerevisiae and established a CRISPR/Cpf1 assisted method for rapid markerless large fragment deletion to facilitate laboratory evolution of geome of S. cerevisiae by rational genetic engineering....