Guanine anchoring : a strategy for specific targeting of a G-quadruplex using short PNA, LNA and DNA molecules

Two separate structural elements of a G-quadruplex (G4), a vacant site and a flanking single-strand, provide an opportunity for specific targeting of a particular G4 structure via dual recognition. Here, we show that a short peptide nucleic acid (PNA) can specifically recognize and bind to a G4 at s...

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Main Authors: Tan, Derrick Jing Yang, Das, Poulomi, Winnerdy, Fernaldo Richtia, Lim, Kah Wai, Phan, Anh Tuân
Other Authors: School of Physical and Mathematical Sciences
Format: Article
Language:English
Published: 2020
Subjects:
PNA
DNA
Online Access:https://hdl.handle.net/10356/142097
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Institution: Nanyang Technological University
Language: English
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spelling sg-ntu-dr.10356-1420972023-02-28T19:48:20Z Guanine anchoring : a strategy for specific targeting of a G-quadruplex using short PNA, LNA and DNA molecules Tan, Derrick Jing Yang Das, Poulomi Winnerdy, Fernaldo Richtia Lim, Kah Wai Phan, Anh Tuân School of Physical and Mathematical Sciences NTU Institute of Structural Biology Science::Chemistry Science::Biological sciences PNA DNA Two separate structural elements of a G-quadruplex (G4), a vacant site and a flanking single-strand, provide an opportunity for specific targeting of a particular G4 structure via dual recognition. Here, we show that a short peptide nucleic acid (PNA) can specifically recognize and bind to a G4 at sub-micromolar affinity based on both G-tetrad vacant site filling and complementary duplex formation. This sequence-guided guanine-anchoring strategy can be further developed for specific targeting of G4 structures using short DNA, LNA and PNA strands. NRF (Natl Research Foundation, S’pore) Accepted version 2020-06-16T00:45:01Z 2020-06-16T00:45:01Z 2020 Journal Article Tan, D. J. Y., Das, P., Winnerdy, F. R., Lim, K. W., & Phan, A. T. (2020). Guanine anchoring : a strategy for specific targeting of a G-quadruplex using short PNA, LNA and DNA molecules. Chemical Communications, 56(44), 5897-5900. doi:10.1039/d0cc01778g 1359-7345 https://hdl.handle.net/10356/142097 10.1039/d0cc01778g 32338660 44 56 5897 5900 en NRF-NRFI2017-09 Chemical Communications © 2020 The Royal Society of Chemistry. All rights reserved. This paper was published in Chemical Communications and is made available with permission of The Royal Society of Chemistry. application/pdf
institution Nanyang Technological University
building NTU Library
continent Asia
country Singapore
Singapore
content_provider NTU Library
collection DR-NTU
language English
topic Science::Chemistry
Science::Biological sciences
PNA
DNA
spellingShingle Science::Chemistry
Science::Biological sciences
PNA
DNA
Tan, Derrick Jing Yang
Das, Poulomi
Winnerdy, Fernaldo Richtia
Lim, Kah Wai
Phan, Anh Tuân
Guanine anchoring : a strategy for specific targeting of a G-quadruplex using short PNA, LNA and DNA molecules
description Two separate structural elements of a G-quadruplex (G4), a vacant site and a flanking single-strand, provide an opportunity for specific targeting of a particular G4 structure via dual recognition. Here, we show that a short peptide nucleic acid (PNA) can specifically recognize and bind to a G4 at sub-micromolar affinity based on both G-tetrad vacant site filling and complementary duplex formation. This sequence-guided guanine-anchoring strategy can be further developed for specific targeting of G4 structures using short DNA, LNA and PNA strands.
author2 School of Physical and Mathematical Sciences
author_facet School of Physical and Mathematical Sciences
Tan, Derrick Jing Yang
Das, Poulomi
Winnerdy, Fernaldo Richtia
Lim, Kah Wai
Phan, Anh Tuân
format Article
author Tan, Derrick Jing Yang
Das, Poulomi
Winnerdy, Fernaldo Richtia
Lim, Kah Wai
Phan, Anh Tuân
author_sort Tan, Derrick Jing Yang
title Guanine anchoring : a strategy for specific targeting of a G-quadruplex using short PNA, LNA and DNA molecules
title_short Guanine anchoring : a strategy for specific targeting of a G-quadruplex using short PNA, LNA and DNA molecules
title_full Guanine anchoring : a strategy for specific targeting of a G-quadruplex using short PNA, LNA and DNA molecules
title_fullStr Guanine anchoring : a strategy for specific targeting of a G-quadruplex using short PNA, LNA and DNA molecules
title_full_unstemmed Guanine anchoring : a strategy for specific targeting of a G-quadruplex using short PNA, LNA and DNA molecules
title_sort guanine anchoring : a strategy for specific targeting of a g-quadruplex using short pna, lna and dna molecules
publishDate 2020
url https://hdl.handle.net/10356/142097
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