Cyclization of a G4-specific peptide enhances its stability and G-quadruplex binding affinity

Cyclization of a G4-specific peptide enhances its stability and G-quadruplex binding affinity

G-quadruplexes(G4) arenon-canonical nucleicacidstructures with important implications in biology. Based on an a-helical fragment of the RHAU helicase that displays high specificity for parallelstranded G-quadrplexes, herein we demonstrate its head-to-tail cyclization by a high-efficiency ligase. The c...

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Bibliographic Details
Main Authors: Ngo, Khac Huy, Yang, Renliang, Das, Poulomi, Nguyen, Giang Kien Truc, Lim, Kah Wai, Tam, James P., Wu, Bin, Phan, Anh Tuân
Other Authors: School of Physical and Mathematical Sciences
Format: Article
Language:English
Published: 2022
Subjects:
Science::Chemistry::Biochemistry
Science::Biological sciences
Guanine Quadruplex
Membrane Protein
Online Access:https://hdl.handle.net/10356/150540
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Institution: Nanyang Technological University
Language: English
Description
Summary:G-quadruplexes(G4) arenon-canonical nucleicacidstructures with important implications in biology. Based on an a-helical fragment of the RHAU helicase that displays high specificity for parallelstranded G-quadrplexes, herein we demonstrate its head-to-tail cyclization by a high-efficiency ligase. The cyclic peptide exhibits superior stability and binding affinity to a G-quadruplex, and can serve as an excellent investigational tool for chemical biology applications

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