Cyclization of a G4-specific peptide enhances its stability and G-quadruplex binding affinity

G-quadruplexes(G4) arenon-canonical nucleicacidstructures with important implications in biology. Based on an a-helical fragment of the RHAU helicase that displays high specificity for parallelstranded G-quadrplexes, herein we demonstrate its head-to-tail cyclization by a high-eﬃciency ligase. The c...

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Main Authors:	Ngo, Khac Huy, Yang, Renliang, Das, Poulomi, Nguyen, Giang Kien Truc, Lim, Kah Wai, Tam, James P., Wu, Bin, Phan, Anh Tuân
Other Authors:	School of Physical and Mathematical Sciences
Format:	Article
Language:	English
Published:	2022
Subjects:	Science::Chemistry::Biochemistry Science::Biological sciences Guanine Quadruplex Membrane Protein
Online Access:	https://hdl.handle.net/10356/150540
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Institution:	Nanyang Technological University
Language:	English

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spelling	sg-ntu-dr.10356-1505402023-02-28T17:10:55Z Cyclization of a G4-specific peptide enhances its stability and G-quadruplex binding affinity Ngo, Khac Huy Yang, Renliang Das, Poulomi Nguyen, Giang Kien Truc Lim, Kah Wai Tam, James P. Wu, Bin Phan, Anh Tuân School of Physical and Mathematical Sciences School of Biological Sciences NTU Institute of Structural Biology Science::Chemistry::Biochemistry Science::Biological sciences Guanine Quadruplex Membrane Protein G-quadruplexes(G4) arenon-canonical nucleicacidstructures with important implications in biology. Based on an a-helical fragment of the RHAU helicase that displays high specificity for parallelstranded G-quadrplexes, herein we demonstrate its head-to-tail cyclization by a high-eﬃciency ligase. The cyclic peptide exhibits superior stability and binding aﬃnity to a G-quadruplex, and can serve as an excellent investigational tool for chemical biology applications Ministry of Education (MOE) Submitted/Accepted version This work was supported by Singapore Ministry of Education (MOE) Academic Research Fund Tier 2 (MOE2018-T2-2-029) to A. T. P. and MOE Academic Research Fund Tier 3 (MOE2016-T3- 1-003) to J. P. T 2022-05-18T02:51:18Z 2022-05-18T02:51:18Z 2020 Journal Article Ngo, K. H., Yang, R., Das, P., Nguyen, G. K. T., Lim, K. W., Tam, J. P., Wu, B. & Phan, A. T. (2020). Cyclization of a G4-specific peptide enhances its stability and G-quadruplex binding affinity. Chemical Communications, 56(7), 1082-1084. https://dx.doi.org/10.1039/C9CC06748E 1359-7345 https://hdl.handle.net/10356/150540 10.1039/C9CC06748E 7 56 1082 1084 en MOE2018-T2-2-029 MOE2016-T3-1-003 Chemical Communications © 2020 The Royal Society of Chemistry. All rights reserved. This paper was published in Chemical Communications and is made available with permission of The Royal Society of Chemistry. application/pdf
institution	Nanyang Technological University
building	NTU Library
continent	Asia
country	Singapore Singapore
content_provider	NTU Library
collection	DR-NTU
language	English
topic	Science::Chemistry::Biochemistry Science::Biological sciences Guanine Quadruplex Membrane Protein
spellingShingle	Science::Chemistry::Biochemistry Science::Biological sciences Guanine Quadruplex Membrane Protein Ngo, Khac Huy Yang, Renliang Das, Poulomi Nguyen, Giang Kien Truc Lim, Kah Wai Tam, James P. Wu, Bin Phan, Anh Tuân Cyclization of a G4-specific peptide enhances its stability and G-quadruplex binding affinity
description	G-quadruplexes(G4) arenon-canonical nucleicacidstructures with important implications in biology. Based on an a-helical fragment of the RHAU helicase that displays high specificity for parallelstranded G-quadrplexes, herein we demonstrate its head-to-tail cyclization by a high-eﬃciency ligase. The cyclic peptide exhibits superior stability and binding aﬃnity to a G-quadruplex, and can serve as an excellent investigational tool for chemical biology applications
author2	School of Physical and Mathematical Sciences
author_facet	School of Physical and Mathematical Sciences Ngo, Khac Huy Yang, Renliang Das, Poulomi Nguyen, Giang Kien Truc Lim, Kah Wai Tam, James P. Wu, Bin Phan, Anh Tuân
format	Article
author	Ngo, Khac Huy Yang, Renliang Das, Poulomi Nguyen, Giang Kien Truc Lim, Kah Wai Tam, James P. Wu, Bin Phan, Anh Tuân
author_sort	Ngo, Khac Huy
title	Cyclization of a G4-specific peptide enhances its stability and G-quadruplex binding affinity
title_short	Cyclization of a G4-specific peptide enhances its stability and G-quadruplex binding affinity
title_full	Cyclization of a G4-specific peptide enhances its stability and G-quadruplex binding affinity
title_fullStr	Cyclization of a G4-specific peptide enhances its stability and G-quadruplex binding affinity
title_full_unstemmed	Cyclization of a G4-specific peptide enhances its stability and G-quadruplex binding affinity
title_sort	cyclization of a g4-specific peptide enhances its stability and g-quadruplex binding affinity
publishDate	2022
url	https://hdl.handle.net/10356/150540
_version_	1759854024975187968

Cyclization of a G4-specific peptide enhances its stability and G-quadruplex binding affinity

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