Identifying purine nucleoside phosphorylase as the target of quinine using cellular thermal shift assay

• Main Authors: Dziekan, Jerzy Michal, Yu, Han, Chen, Dan, Dai, Lingyun, Wirjanata, Grennady, Larsson, Andreas, Prabhu, Nayana, Sobota, Radoslaw Mikolaj, Bozdech, Zbynek, Nordlund, Pär
• Other Authors: School of Biological Sciences
• Format: Article
• Language: English
• Published: 2021
• Subjects: Science::Biological sciences; Biological Assay; Mefloquine; Molecular
• Online Access: https://hdl.handle.net/10356/152477
• Institution: Nanyang Technological University

Mechanisms of action (MoAs) have been elusive for most antimalarial drugs in clinical use. Decreasing responsiveness to antimalarial treatments stresses the need for a better resolved understanding of their MoAs and associated resistance mechanisms. In the present work, we implemented the cellular t...